Parihar Tasmeen J, Naik Madeeha, Mehraj Shafqat, Inam Ul Haq Syed, Perveen Maqsooda, Malla Ishfaq Ahmed, Abid Taniya, Gul Nadia, Masoodi Khalid Z
Transcriptomics Laboratory (K-Lab), Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu and Kashmir, 190025, India.
College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu and Kashmir, 190025, India.
Sci Rep. 2025 Jan 31;15(1):3855. doi: 10.1038/s41598-025-87668-3.
The objective of this study was to identify and characterize the fungal pathogens responsible for wilt diseases in solanaceous crops, specifically tomato, brinjal, and chili, in the Kashmir valley. Through both morphological and molecular analyses, including DNA barcoding of the ITS, TEF, RPB1, and RPB2 genomic regions, Fusarium incarnatum and Fusarium avenaceum were identified as the primary causal agents of wilt in tomato and brinjal, and chili, respectively. Pathogenicity tests confirmed the virulence of these pathogens, with typical wilt symptoms observed upon inoculation. This represents the first report of F. incarnatum and F. avenaceum as wilt pathogens in solanaceous crops in India. Phylogenetic analysis further confirmed the genetic variability of these pathogens, revealing their expanding host range. The findings underscore the growing adaptability of these Fusarium species to diverse agricultural systems and highlight the urgent need for targeted disease management strategies to mitigate the significant yield losses caused by Fusarium wilt in solanaceous vegetable production.
本研究的目的是鉴定和表征克什米尔山谷茄科作物（特别是番茄、茄子和辣椒）枯萎病的致病真菌病原体。通过形态学和分子分析，包括对ITS、TEF、RPB1和RPB2基因组区域进行DNA条形码分析，分别鉴定出肉色镰刀菌和燕麦镰刀菌是番茄、茄子和辣椒枯萎病的主要病原体。致病性测试证实了这些病原体的毒力，接种后观察到典型的枯萎症状。这是印度首次报道肉色镰刀菌和燕麦镰刀菌作为茄科作物枯萎病的病原体。系统发育分析进一步证实了这些病原体的遗传变异性，揭示了它们不断扩大的寄主范围。研究结果强调了这些镰刀菌物种对不同农业系统的适应能力不断增强，并突出了迫切需要有针对性的病害管理策略，以减轻茄科蔬菜生产中由枯萎病造成的重大产量损失。
