牙科技术密封剂：材料性能和抗生物膜潜力的体外评估

Technological dental sealants: in vitro evaluation of material properties and antibiofilm potential.

作者信息

de Jesus Witalo Pereira, de Almeida Queiroz Ferreira Luiza, da Silva Washington Henrique Themoteo, Belém Fernanda Vieira, Turrioni Ana Paula, de Magalhães Cláudia Silami, Madeira Mila Fernandes Moreira, Diniz Ivana Márcia Alves, Paschoal Marco Aurelio Benini

机构信息

Dental School, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil.

Dental School, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.

出版信息

BMC Oral Health. 2025 Feb 1;25(1):171. doi: 10.1186/s12903-024-05303-5.

DOI:10.1186/s12903-024-05303-5

PMID:39891129

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11786408/

Abstract

BACKGROUND

Due to the launch of new self-etching and self-adhesive sealing materials combined with the release of bioactive ions in the dental market, this in vitro study aimed to compare physical values of surface roughness (RS), vickers microhardness (VM), compositional energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), microbiological tests (inhibition halo-HI and surface microbial adhesion-MAS) and mechanical microshear bond strength (MBS).

METHODS

The following groups were analyzed: Beautisealant® self-etching sealant (Shofu)(G1), FluroShield® conventional sealant (Dentsply)(G2- Control Group), Flow Constic® self-adhesive/self-etching resin (DMG)(G3) and Beautifil Flow Plus F03® conventional resin (Shofu)(G4). For the physical and compositional tests, specimens were molded and submitted to finishing/polishing in a metallographic polisher. RS and VM were measured through 5 readings. For the HI test, specimens were exposed to S. mutans for 24 h in brain-heart infusion (BHI) agar under microaerophilic conditions, with the diameter of the HI (mm) measured by digital caliper. For the MAS test, a bacterial biofilm of S. mutans was induced for five days in BHI broth + 5% sucrose and measurement of CFU/ml (log from serial dilutions). For the MBS test, specimens were made in bovine enamel with the addition of the experimental group FluroShield® (Dentsply) + Single Bond Universal® adhesive (3 M)(G5) and measured in a universal testing machine. Data were submitted to ANOVA and Tukey's post hoc tests (p < 0.05) using the SPSS software.

RESULTS

Different compositions proven in the EDS test were not determinants of the promotion of different RS: G1 = 0,19 (± 0,07), G2 = 0,15 (± 0,053), G3 = 0,13 (± 0,046), G4 = 0,14 (± 0,056); HI: G1 = 17,7 (± 1,36), G2 = 18,7 (± 3,47), G3 = 18,9 (± 4,87), G4 = 17,9 (± 3,61) and MAS: G1 = 5,22 (± 0,12), G2 = 5,15 (± 0,15), G3 = 5,12 (± 0,20), G4 = 5,06 (± 0,24). However, statistically influenced the values of VM: G1 = 16,71 (± 1,93), G2 = 16,28 (± 4,91), G3 = 26,11 (± 3,46), G4 = 37.9 (± 4.87), promoted lesser bacterial adhesion (G4 = 5.06 ± 0.24), and demonstrated fluoride ion only in the G4. Higher MBS was found in the conventional materials G5 = 8.38 (± 2.05) and G2 = 6.28 (± 1.35).

CONCLUSIONS

Bioactive materials and self-adhesive/self-etching for sealing pits and fissures did not demonstrate superior mechanical and antimicrobial properties performance compared to conventional techniques.

摘要

背景

由于牙科市场上新的自酸蚀和自粘接封闭材料的推出以及生物活性离子的释放，本体外研究旨在比较表面粗糙度（RS）、维氏显微硬度（VM）、能谱分析（EDS）、扫描电子显微镜（SEM）、微生物学测试（抑菌圈 - HI和表面微生物黏附 - MAS）以及机械微剪切粘结强度（MBS）的物理值。

方法

分析以下几组：Beautisealant®自酸蚀封闭剂（松风公司）（G1）、FluroShield®传统封闭剂（登士柏公司）（G2 - 对照组）、Flow Constic®自粘接/自酸蚀树脂（DMG公司）（G3）以及Beautifil Flow Plus F03®传统树脂（松风公司）（G4）。对于物理和成分测试，将标本成型并在金相抛光机中进行精加工/抛光。通过5次读数测量RS和VM。对于HI测试，将标本在微需氧条件下于脑心浸液（BHI）琼脂中暴露于变形链球菌24小时，用数字卡尺测量HI的直径（mm）。对于MAS测试，在BHI肉汤 + 5%蔗糖中诱导变形链球菌的细菌生物膜5天，并测量CFU/ml（系列稀释的对数）。对于MBS测试，在牛牙釉质中制备标本，并添加实验组FluroShield®（登士柏公司）+ Single Bond Universal®粘合剂（3M公司）（G5），并在万能试验机上进行测量。使用SPSS软件将数据提交给方差分析和Tukey事后检验（p < 0.05）。

结果

EDS测试中证明的不同成分不是促进不同RS的决定因素：G1 = 0.19（±0.07），G2 = 0.15（±0.053），G3 = 0.13（±0.046），G4 = 0.14（±0.056）；HI：G1 = 17.7（±1.36），G2 = 18.7（±3.47），G3 = 18.9（±4.87），G4 = 17.9（±3.61）以及MAS：G1 = 5.22（±0.12），G2 = 5.15（±0.15），G3 = 5.12（±0.20），G4 = 5.06（±0.24）。然而，在统计学上影响了VM的值：G1 = 16.71（±1.93），G2 = 16.28（±4.91），G3 = 26.11（±3.46），G4 = 37.9（±4.87），促进较少的细菌黏附（G4 = 5.06 ± 0.24），并且仅在G4中显示出氟离子。在传统材料G5 = 8.38（±2.05）和G2 = 6.28（±1.35）中发现了更高的MBS。