Hoffman D R
J Allergy Clin Immunol. 1985 May;75(5):606-10. doi: 10.1016/0091-6749(85)90037-5.
RAST disks were prepared with purified proteins from the venoms of Vespula squamosa, Dolichovespula maculata, and Polistes exclamans and tested against a panel of sera obtained from patients allergic to each of the venoms. By the use of several criteria, IgE binding was strongest to antigen 5 of V. squamosa and phospholipase of P. exclamans. Hyaluronidase from Polistes had little IgE binding activity. All five proteins tested from D. maculata bound IgE antibodies strongly. Linear correlation analysis suggested that all of the proteins were distinct allergens in each of the venoms. A variety of patterns of response were observed for individual sera with each of the venoms.
用来自黄胡蜂、黄斑胡蜂和惊人文胡蜂毒液的纯化蛋白制备了放射变应原吸附试验（RAST）盘，并针对从对每种毒液过敏的患者获得的一组血清进行检测。通过使用多种标准，免疫球蛋白E（IgE）与黄胡蜂的抗原5和惊人文胡蜂的磷脂酶结合最强。来自文胡蜂的透明质酸酶几乎没有IgE结合活性。从黄斑胡蜂检测的所有五种蛋白质都强烈结合IgE抗体。线性相关分析表明，所有蛋白质在每种毒液中都是不同的变应原。对于每种毒液的个体血清，观察到了多种反应模式。
