Reisman R E, Wypych J I, Mueller U R, Grant J A
J Allergy Clin Immunol. 1982 Oct;70(4):281-7. doi: 10.1016/0091-6749(82)90064-1.
The crossantigenicity of Polistes venom with other vespid venoms was examined with rabbit and human antisera. Venom preparations from various Polistes species were obtained by electrical stimulation of individual insects and venom sac dissection. Rabbit antibodies were raised to the venom (P. apachus) and venom sac extract (P. exclamans). Human antisera were obtained from patients allergic to Polistes and other vespid venoms. The venom appeared to be more potent than the venom sac preparations in reactions with rabbit IgG and human IgE antibodies. Among the Polistes species, P. exclamans, P. instablis, and P. apachus venoms showed several lines of precipitation with rabbit antisera, and P. annularis and P. fuscatus venoms only one line, suggesting quantitative or qualitative antigenic differences. In RAST analysis, most sera reacted equally to all Polistes species but occasional exceptions were noted, again suggesting differences in venom allergens. P. exclamans-coupled discs gave the most consistent results. In gel diffusion experiments, there was no crossreactivity between Polistes and yellow jacket venoms and only limited crossreactivity between Polistes and hornet venoms. Patients sensitive to Polistes venom showed varying degrees of reactivity to yellow jacket and hornet venoms in RAST analysis. Patients sensitive to other vespid venoms also showed varying degrees os sensitivity to Polistes venom. Polistes venom appears to contain a genus-unique antigen (allergen). In addition, there appear to be some crossreacting antigens in Polistes and other vespid venoms but to a much lesser degree than found previously in the analysis of the relationship of yellow jacket and hornet venoms.
用兔和人抗血清检测了胡蜂毒液与其他黄蜂毒液的交叉抗原性。通过对单个昆虫进行电刺激和解剖毒囊获得了来自不同胡蜂种类的毒液制剂。用毒液（阿帕奇胡蜂）和毒囊提取物（惊胡蜂）制备兔抗体。人抗血清取自对胡蜂和其他黄蜂毒液过敏的患者。在与兔IgG和人IgE抗体的反应中，毒液似乎比毒囊制剂更具效力。在胡蜂种类中，惊胡蜂、不稳定胡蜂和阿帕奇胡蜂的毒液与兔抗血清呈现出多条沉淀线，而环纹胡蜂和暗褐胡蜂的毒液只有一条沉淀线，这表明存在定量或定性的抗原差异。在放射变应原吸附试验分析中，大多数血清对所有胡蜂种类的反应相同，但也有偶尔的例外情况，这再次表明毒液过敏原存在差异。惊胡蜂偶联的圆盘给出的结果最一致。在凝胶扩散试验中，胡蜂毒液与黄胡蜂毒液之间没有交叉反应，胡蜂毒液与大黄蜂毒液之间只有有限的交叉反应。对胡蜂毒液敏感的患者在放射变应原吸附试验分析中对黄胡蜂和大黄蜂毒液表现出不同程度的反应。对其他黄蜂毒液敏感的患者对胡蜂毒液也表现出不同程度的敏感性。胡蜂毒液似乎含有一种属特异性抗原（过敏原）。此外，胡蜂毒液与其他黄蜂毒液中似乎存在一些交叉反应抗原，但程度远低于先前对黄胡蜂和大黄蜂毒液关系分析中所发现的程度。
