一种用于亚单位疫苗抗原病毒体展示的模块化方案。
A modular protocol for virosome display of subunit vaccine antigens.
作者信息
Rosado Victoria C, Adams Lindsey, Yousif Ashraf S, Sangesland Maya, Ronsard Larance, Okonkwo Vintus, McCarthy Caitlin, Alexander Caroline, Irvine Darrell, Lingwood Daniel
机构信息
The Ragon Institute of Mass General, The Massachusetts Institute of Technology and Harvard University, 600 Main Street, Cambridge, MA 02139, USA.
University of Virginia, School of Medicine, Beirne B. Carter Center for Immunology Research, 345 Crispell Dr., Charlottesville, VA 22908, USA.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103610. doi: 10.1016/j.xpro.2025.103610. Epub 2025 Jan 31.
Antigen array increases B cell receptor (BCR) triggering and the titer of antibodies elicited by subunit vaccines. Here, we present a protocol for multivalent antigen display by synthetic virosomes: preformed liposomes bearing glycoprotein spike proteins from enveloped viruses. We describe how to customize lipid stoichiometry within preformed liposomes and attach user-defined antigens via covalent and/or non-covalent interactions. In addition to generating vaccine research tools, this protocol demonstrates how two-dimensional membrane array resolves and activates exceptionally weak but critical virus-receptor interactions.
抗原阵列可增强B细胞受体（BCR）触发作用以及亚单位疫苗引发的抗体滴度。在此，我们展示了一种通过合成病毒体进行多价抗原展示的方案：预先形成的脂质体带有来自包膜病毒的糖蛋白刺突蛋白。我们描述了如何在预先形成的脂质体内定制脂质化学计量，并通过共价和/或非共价相互作用连接用户定义的抗原。除了生成疫苗研究工具外，该方案还展示了二维膜阵列如何解析并激活异常微弱但关键的病毒-受体相互作用。
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