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使用非侵入性微超声成像和解剖 dissection 研究小鼠肠系膜上动脉的方案。 （注：这里“dissection”直接翻译为“解剖”，但感觉在该语境下用“解剖分离”之类表述会更准确些，不过按照要求未添加其他解释说明）

Protocol for studying the superior mesenteric artery in mice using non-invasive micro-ultrasound imaging and anatomic dissection.

作者信息

Chen Haiping, Jiang Yangjing, Huo Huanhuan, Zhou Guo, Liang Min, Huang Yijie, Hu Yunwen, Zhang Ziwei, He Ben, Cai Zhaohua

机构信息

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103606. doi: 10.1016/j.xpro.2025.103606. Epub 2025 Jan 30.

DOI:10.1016/j.xpro.2025.103606
PMID:39891916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11834953/
Abstract

The superior mesenteric artery (SMA), originating from the abdominal aorta, provides blood supply for the spleen, pancreas, intestine, and part of the colon. Here, we present a protocol for studying the SMA in the same mouse by combining non-invasive micro-ultrasound imaging and SMA dissection. We then detail procedures for tissue preparation, sectioning, and histological analysis. By allowing accurate identification of the SMA by ultrasound and anatomical dissection, this protocol can provide insights into SMA-related diseases in animal models.

摘要

肠系膜上动脉（SMA）起源于腹主动脉，为脾脏、胰腺、肠道及部分结肠提供血液供应。在此，我们介绍一种通过结合非侵入性微超声成像和SMA解剖来在同一只小鼠中研究SMA的方案。然后，我们详细说明了组织制备、切片和组织学分析的程序。通过超声和解剖学解剖能够准确识别SMA，该方案可为动物模型中与SMA相关的疾病提供见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/7cde4cc50150/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/62686cd929da/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/ec60a123fb9d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/83cfd72a4a7c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/85f9210b7746/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/c90a30525800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/5de894218940/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/6e9d1c2d08ab/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/4db3ed2e9ebd/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/bb9a919c74c2/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/817574498ca7/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/7cde4cc50150/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/62686cd929da/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/ec60a123fb9d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/83cfd72a4a7c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/85f9210b7746/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/c90a30525800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/5de894218940/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/6e9d1c2d08ab/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/4db3ed2e9ebd/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/bb9a919c74c2/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/817574498ca7/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d4/11834953/7cde4cc50150/gr10.jpg

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本文引用的文献

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European guidelines on chronic mesenteric ischaemia - joint United European Gastroenterology, European Association for Gastroenterology, Endoscopy and Nutrition, European Society of Gastrointestinal and Abdominal Radiology, Netherlands Association of Hepatogastroenterologists, Hellenic Society of Gastroenterology, Cardiovascular and Interventional Radiological Society of Europe, and Dutch Mesenteric Ischemia Study group clinical guidelines on the diagnosis and treatment of patients with chronic mesenteric ischaemia.欧洲慢性肠系膜缺血指南 - 联合欧洲胃肠病学、欧洲胃肠病学、内镜和营养学会、欧洲胃肠道和腹部放射学会、荷兰肝胆病学会、希腊胃肠病学会、心血管和介入放射学会和荷兰肠系膜缺血研究小组的临床指南，用于诊断和治疗慢性肠系膜缺血患者。
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Superior mesenteric artery calcification is associated with cardiovascular risk factors, systemic calcified atherosclerosis, and increased mortality.肠系膜上动脉钙化与心血管危险因素、全身性钙化性动脉粥样硬化和死亡率增加有关。
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