Rong Xiaodi, Liu Caiyun, Hou Fengli, Zhao Xin, Wang Zhongpeng, Zhu Baocun
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
Food Chem. 2025 May 15;474:143045. doi: 10.1016/j.foodchem.2025.143045. Epub 2025 Jan 23.
The extensive use of glyphosate has been a severe threat to the environment and human health, which highlights the importance of the detection of glyphosate in various environmental and food samples. Herein, a novel fluorescent probe (DT-Gly) was constructed for the detection of glyphosate. DT-Gly and glyphosate binding to copper ions (Cu) is competitive. Therefore, the increased glyphosate will decrease DT-Gly binds to Cu. Accordingly, the absorbance (at 507 nm) and fluorescence (at 580 nm) intensity of DT-Gly showed excellent linear relationships with glyphosate concentrations (0-20 μM). Moreover, the detection limits of DT-Gly for Cu and glyphosate were 94 nM and 71 nM, respectively. DT-Gly was successfully applied to detect glyphosate in food samples and water samples. We further constructed a convenient platform for the detection of glyphosate by analyzing the changes in RGB values of the probe solution and test strips using a smartphone.
草甘膦的广泛使用对环境和人类健康构成了严重威胁，这凸显了在各种环境和食品样品中检测草甘膦的重要性。在此，构建了一种新型荧光探针（DT-Gly）用于检测草甘膦。DT-Gly与草甘膦对铜离子（Cu）的结合具有竞争性。因此，草甘膦含量增加会减少DT-Gly与Cu的结合。相应地，DT-Gly的吸光度（507nm处）和荧光强度（580nm处）与草甘膦浓度（0-20μM）呈现出良好的线性关系。此外，DT-Gly对Cu和草甘膦的检测限分别为94nM和71nM。DT-Gly已成功应用于食品样品和水样中草甘膦的检测。我们还通过使用智能手机分析探针溶液和试纸条的RGB值变化，构建了一个便捷的草甘膦检测平台。
