Cole S P, Vreeken E H, Roder J C
J Immunol Methods. 1985 Apr 22;78(2):271-8. doi: 10.1016/0022-1759(85)90085-7.
Four human X human hybridomas were adapted to growth in serum-free medium consisting of RPMI 1640 supplemented with bovine serum albumin and transferrin (BSA/Tf medium). Production of specific monoclonal antibodies was maintained for more than 2 months. Although the maximal cell density achieved was lower than that in serum-supplemented medium, immunoglobulin production was similar or higher when results were expressed on a per viable cell basis. Thus it is feasible to grow human X human hybridomas in serum-free culture and it is possible that this will become the method of choice for large scale production of human monoclonal antibodies.
将四个人源人杂交瘤细胞适应于在由添加了牛血清白蛋白和转铁蛋白的RPMI 1640组成的无血清培养基（BSA/Tf培养基）中生长。特异性单克隆抗体的产生维持了两个多月。尽管达到的最大细胞密度低于补充血清的培养基中的密度，但以每活细胞为基础表达结果时，免疫球蛋白的产生相似或更高。因此，在无血清培养中培养人源人杂交瘤细胞是可行的，并且这有可能成为大规模生产人单克隆抗体的首选方法。
