Glassy M C, Peters R E, Mikhalev A
Biotherapeutics, Inc., La Jolla, California 92037.
In Vitro Cell Dev Biol. 1987 Nov;23(11):745-9. doi: 10.1007/BF02623674.
Human-human hybridomas derived from fusing lymph node lymphocytes with UC 729-6 were adapted to grow in commercially available serum-free medium and were compared with serum-supplemented [10% fetal bovine serum (FBS)] cultures. Over a 6-d period, no significant changes occurred in the growth of the cells in 10% FBS or serum-free medium. In cultures supplemented with 10% FBS more total proteins were secreted than in serum-free cultures. However, there was an enhanced secretion of three- to four-fold of both immunoreactive human IgG and IgM under serum-free conditions compared to serum-supplemented conditions. Serum-free conditions may provide the appropriate milieu for the increased level of Ig secretion from human hybridomas derived from UC 729-6 in that there are no inhibitors that may be present in serum.
将淋巴结淋巴细胞与UC 729-6融合得到的人-人杂交瘤适应于在市售无血清培养基中生长，并与添加血清的[10%胎牛血清（FBS）]培养物进行比较。在6天的时间里，10% FBS或无血清培养基中细胞的生长没有显著变化。在添加10% FBS的培养物中分泌的总蛋白比无血清培养物中多。然而，与添加血清的条件相比，在无血清条件下免疫反应性人IgG和IgM的分泌增加了三到四倍。无血清条件可能为源自UC 729-6的人杂交瘤增加Ig分泌水平提供合适的环境，因为血清中可能不存在抑制剂。
