State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong, 266237, China.
Sci Data. 2024 Jun 4;11(1):576. doi: 10.1038/s41597-024-03423-9.
Exopalaemon carinicauda, a eurythermal and euryhaline shrimp, contributes one third of the total biomass production of polyculture ponds in eastern China and is considered as a potential ideal experimental animal for research on crustaceans. We conducted a high-quality chromosome-level genome assembly of E. carinicauda combining PacBio HiFi and Hi-C sequencing data. The total assembly size was 5.86 Gb, with a contig N50 of 235.52 kb and a scaffold N50 of 138.24 Mb. Approximately 95.29% of the assembled sequences were anchored onto 45 pseudochromosomes. BUSCO analysis revealed that 92.89% of 1,013 single-copy genes were highly conserved orthologs. A total of 44, 288 protein-coding genes were predicted, of which 70.53% were functionally annotated. Given its high heterozygosity (2.62%) and large proportion of repeat sequences (71.49%), it is one of the most complex genome assemblies. This chromosome-scale genome will be a valuable resource for future molecular breeding and functional genomics research on E. carinicauda.
脊尾白虾(Exopalaemon carinicauda)是一种广温性和广盐性虾类,其生物量约占中国东部多营养养殖池塘的三分之一,被认为是甲壳类动物研究的理想实验动物。我们结合 PacBio HiFi 和 Hi-C 测序数据,对脊尾白虾进行了高质量的染色体水平基因组组装。总组装大小为 5.86Gb,contig N50 为 235.52kb,scaffold N50 为 138.24Mb。大约 95.29%的组装序列锚定在 45 条假染色体上。BUSCO 分析表明,1013 个单拷贝基因中有 92.89%是高度保守的直系同源物。共预测到 44288 个蛋白质编码基因,其中 70.53%具有功能注释。由于其高杂合性(2.62%)和大量重复序列(71.49%),这是最复杂的基因组组装之一。该染色体水平的基因组将成为脊尾白虾未来分子育种和功能基因组学研究的宝贵资源。