Prins Rianne C, Billerbeck Sonja
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, the Netherlands.
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, the Netherlands; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
STAR Protoc. 2025 Mar 21;6(1):103611. doi: 10.1016/j.xpro.2025.103611. Epub 2025 Jan 31.
Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis. For complete details on the use and execution of this protocol, please refer to Prins et al..
杀伤性酵母会产生抗菌蛋白，通常还会产生一种自我保护免疫因子。在此，我们提供了一种分析酿酒酵母中毒性、免疫性和自杀性表型的方案。我们描述了通过晕圈试验评估毒性、通过点样试验评估自杀性以及使用微量滴定板生长试验评估免疫性的步骤。我们详细说明了酵母培养、进行试验和数据分析的程序。有关此方案的使用和执行的完整详细信息，请参考普林斯等人的研究。
