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自由基S-腺苷甲硫氨酸酶WprB催化前体肽上色氨酸C5和精氨酸Cγ之间的均匀交联拓扑结构。

Radical SAM Enzyme WprB Catalyzes Uniform Cross-Link Topology between Trp-C5 and Arg-Cγ on the Precursor Peptide.

作者信息

Khan Abujunaid Habib, Haedar Jabal Rahmat, Kiselov Vic, Romanuks Viktors, Smits Gints, Donadio Stefano, Phan Chin-Soon

机构信息

Latvian Institute of Organic Synthesis, Aizkraukles Street 21, LV-1006 Riga, Latvia.

NAICONS Srl, 20139 Milan, Italy.

出版信息

ACS Chem Biol. 2025 Feb 21;20(2):259-265. doi: 10.1021/acschembio.4c00693. Epub 2025 Feb 2.

DOI:10.1021/acschembio.4c00693
PMID:39893661
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11851443/
Abstract

Cross-link containing products from ribosomally synthesized and post-translationally modified peptides (RiPPs) are generated by radical SAM enzymes (rSAM). Here, we bioinformatically expanded rSAM enzymes based on the known families StrB, NxxcB, WgkB, RrrB, TqqB and GggB. Through functional studies in , the newly identified enzyme WprB from sp. psl was found to catalyze formation of a cross-link between Trp-C5 and Arg-Cγ at three WPR motifs on the precursor peptide WprA. This represents the first report of this type of cross-link by rSAM enzymes.

摘要

核糖体合成及翻译后修饰肽（RiPPs）中含交联的产物由自由基S-腺苷甲硫氨酸酶（rSAM）生成。在此，我们基于已知的StrB、NxxcB、WgkB、RrrB、TqqB和GggB家族对rSAM酶进行了生物信息学扩展。通过对[具体物种]的功能研究，发现从[具体物种]psl中新鉴定出的酶WprB可催化前体肽WprA上三个WPR基序处色氨酸-C5与精氨酸-Cγ之间交联的形成。这是rSAM酶催化此类交联的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/9da1badb44c4/cb4c00693_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/040839c9b352/cb4c00693_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/cf27a00ad705/cb4c00693_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/ac4634d022dc/cb4c00693_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/9da1badb44c4/cb4c00693_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/040839c9b352/cb4c00693_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/cf27a00ad705/cb4c00693_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/ac4634d022dc/cb4c00693_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b80/11851443/9da1badb44c4/cb4c00693_0004.jpg

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