Zhou Yanyu, Liu Fucun, Yuan Meile, Liu Xianhua, Li Qipei, Zhao Hongwei
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China; Center for Eco-Environment Restoration of Hainan Province, School of Ecology, Hainan University, Haikou 570228, China; Hainan International Joint Research Center for Coral Reef Ecology, Hainan University, Haikou 570228, China.
School of Environmental Science and Engineering, Tianjin University, Yaguan Road, Tianjin 300350, China.
J Hazard Mater. 2025 May 5;488:137389. doi: 10.1016/j.jhazmat.2025.137389. Epub 2025 Jan 26.
Ocean warming threatens the health of corals globally, and superimposed coastal environmental pollution can result in severe and irreversible coral bleaching. However, the responses of the coral symbiont Symbiodiniaceae to multiple stresses remain largely unknown. This study investigated the response of the coral symbiotic algae Cladocopium sp. to short-term exposure (4 days) to an environmentally relevant concentration (1 μg L) of the photosystem II (PSII) herbicide prometryn under heat stress (32 ℃) through physiological and omic analyses. These results showed that co-stress affected the photosynthetic efficiency of Cladocopium sp. negatively. Overproduction of reactive oxygen species and subsequent oxidative stress under co-stress activated distinct regulatory pathways in Cladocopium sp. Transcriptomic and proteomic analyses revealed that prometryn exacerbated heat stress-induced photosystem damage and reduced the regulatory capacity of Cladocopium sp. Moreover, co-stress disrupted energy metabolism, and further impaired nitrogen assimilation and nutrient transfer processes, potentially compromising the symbiotic potential between corals and Symbiodiniaceae. In summary, this study offers a valuable insight into understanding the molecular responses of Symbiodiniaceae to thermal and prometryn co-stress. It helps uncover the potential toxicity mechanisms induced by herbicide on coral symbionts in the context of climate change.
海洋变暖威胁着全球珊瑚的健康，叠加的沿海环境污染会导致严重且不可逆转的珊瑚白化。然而，珊瑚共生体共生藻对多种胁迫的反应仍 largely unknown。本研究通过生理和组学分析，调查了珊瑚共生藻枝管藻属（Cladocopium sp.）在热胁迫（32℃）下短期暴露（4天）于环境相关浓度（1μg/L）的光系统II（PSII）除草剂扑草净后的反应。这些结果表明，复合胁迫对枝管藻属的光合效率产生了负面影响。复合胁迫下活性氧的过量产生及随后的氧化应激激活了枝管藻属中不同的调控途径。转录组学和蛋白质组学分析表明，扑草净加剧了热胁迫诱导的光系统损伤，并降低了枝管藻属的调控能力。此外，复合胁迫扰乱了能量代谢，进一步损害了氮同化和养分转移过程，可能会损害珊瑚与共生藻之间的共生潜力。总之，本研究为理解共生藻对热和扑草净复合胁迫的分子反应提供了有价值的见解。它有助于揭示在气候变化背景下除草剂对珊瑚共生体诱导的潜在毒性机制。
