Bjedov Dora, Turić Nataša, Mikuška Alma, Vignjević Goran, Kovačić Lucija Sara, Pavičić Ana Marija, Toth Jakeljić Leontina, Velki Mirna
Department of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, Croatia.
Department of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, Croatia; Teaching Institute of Public Health Osijek-baranja County, Drinska 8, 31000 Osijek, Croatia.
Aquat Toxicol. 2025 Feb;279:107258. doi: 10.1016/j.aquatox.2025.107258. Epub 2025 Jan 23.
The present study assessed Cybister lateralimarginalis as a model species for ecotoxicological studies of metal(loid) bioaccumulation in freshwater ecosystems, by optimising protocols and analysing biomarker activities and metal(loid) concentrations from different sites and investigating their association. To accomplish this, levels of arsenic (As), selenium (Se), cadmium (Cd), mercury (Hg), and lead (Pb) were analysed, as well as a set of biomarkers were evaluated, esterase activity and oxidative stress biomarkers: fluorescence- and glutathione-based. C. lateralimarginalis were sampled in the spring/summer of 2023 along the Sava, Drava and Danube floodplains in Croatia. C. lateralimarginalis samples from Sava floodplain (Mužilovčica) showed altered carboxylesterase (CES) activity, lower glutathione (GSH) levels, and elevated Hg and As concentrations. The Weighted Pollution Index (WPI) indicated moderate pollution at the Sava floodplain, possibly due to agricultural or petrochemical impacts. At the Drava floodplain (Podravlje), higher glutathione S-transferase (GST) activity and elevated Cd, Hg, and As concentrations were observed, likely from soil accumulation, while Stara Drava beetles had lower GSH levels, suggesting agricultural impacts. Despite this, the Drava floodplain showed the lowest WPI. Higher GST activity was noted at the Danube floodplain (Kopački rit and Topoljski Dunavac), with reactive oxygen species (ROS) concentrations (Kopački rit and Podunavlje) possibly reflecting agricultural practices or illegal hunting. The present research successfully applied C. lateralimarginalis as a bioindicator species for assessing metal(loid)s in different environments, highlighting its reliability as an abundant apex predator, emphasizing the significance of integrating biomarker response evaluation into pollutant monitoring for efficient environmental risk assessment in freshwater ecosystems.
本研究通过优化实验方案、分析不同地点的生物标志物活性和金属（类金属）浓度，并研究它们之间的关联，评估了宽边沼梭（Cybister lateralimarginalis）作为淡水生态系统中金属（类金属）生物累积生态毒理学研究的模式物种。为实现这一目标，分析了砷（As）、硒（Se）、镉（Cd）、汞（Hg）和铅（Pb）的含量，并评估了一组生物标志物，即酯酶活性和氧化应激生物标志物：基于荧光和谷胱甘肽的生物标志物。2023年春夏，在克罗地亚的萨瓦河、德拉瓦河和多瑙河河漫滩采集了宽边沼梭样本。来自萨瓦河河漫滩（穆日洛夫契察）的宽边沼梭样本显示，羧酸酯酶（CES）活性改变、谷胱甘肽（GSH）水平降低，以及汞和砷浓度升高。加权污染指数（WPI）表明萨瓦河河漫滩受到中度污染，这可能是由于农业或石化影响。在德拉瓦河河漫滩（波德拉夫列），观察到较高的谷胱甘肽S-转移酶（GST）活性以及镉、汞和砷浓度升高，这可能源于土壤积累，而旧德拉瓦河的甲虫谷胱甘肽水平较低，表明受到了农业影响。尽管如此，德拉瓦河河漫滩的WPI最低。多瑙河河漫滩（科帕čki里特和托波尔斯基多瑙瓦茨）的GST活性较高，活性氧物种（ROS）浓度（科帕čki里特和波杜纳夫列）可能反映了农业活动或非法捕猎情况。本研究成功地将宽边沼梭作为生物指示物种用于评估不同环境中的金属（类金属），突出了其作为丰富的顶级捕食者的可靠性，强调了将生物标志物反应评估纳入污染物监测对于淡水生态系统有效环境风险评估的重要性。
