Okawa Hiroaki, Yukiyama Norihiro, Yamato Osamu, Goto Akira, Widodo Oky Setyo, Fushimi Yasuo, Takagi Mitsuhiro
Guardian Co., Ltd., Kagoshima 890-0033, Japan.
Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
J Reprod Dev. 2025 Apr 14;71(2):93-98. doi: 10.1262/jrd.2024-092. Epub 2025 Jan 31.
In Japanese Black (JB) cattle, the number of transferable embryos produced after superovulation is crucial for the economic success of embryo production for both farmers and veterinarians. Anti-Müllerian hormone (AMH) has emerged as a key reproductive marker for predicting the number of embryos produced in vivo and oocytes retrieved through transvaginal pickup. This study investigated the relationship between AMH, inflammatory markers, including serum amyloid A (SAA) and albumin/globulin (A/G) ratio, and the number of embryos recovered and transferable after superovulation in JB cows. A total of 96 JB donor cows underwent artificial insemination after superovulation, and embryo recovery was performed 7 days later. Embryos recovered were classified based on the International Embryo Technology Society criteria, wherein "transferable embryos" included those with codes 1 or 2, while "total embryos" included transferable embryos as well as those with codes 3 and 4. Blood samples collected during embryo recovery were used to measure serum AMH, SAA, and A/G ratios. When grouped by AMH quartiles, the high-AMH and middle-high-AMH groups produced significantly more total embryos compared to the low-AMH group. The total number of embryos increased with higher AMH levels (r = 0.3336, P = 0.0009). Correlation analysis revealed associations between AMH, α-globulin and SAA. Additionally, a significant positive correlation was observed between total and transferable embryos (r = 0.6339, P < 0.0001) and between AMH and the yield ratio (r = 0.25583, P = 0.0119). These findings confirm that AMH concentration is a valuable reproductive marker for predicting the total and transferable embryos produced by JB donor cows.
在日本黑毛（JB）牛中，超排后可移植胚胎的数量对于农民和兽医而言，在胚胎生产的经济成功方面至关重要。抗苗勒管激素（AMH）已成为预测体内产生的胚胎数量以及经阴道采卵获取的卵母细胞数量的关键生殖标志物。本研究调查了日本黑毛母牛超排后AMH、包括血清淀粉样蛋白A（SAA）和白蛋白/球蛋白（A/G）比值在内的炎症标志物与回收及可移植胚胎数量之间的关系。总共96头日本黑毛供体母牛在超排后进行人工授精，并在7天后进行胚胎回收。回收的胚胎根据国际胚胎技术协会标准进行分类，其中“可移植胚胎”包括代码为1或2的胚胎，而“总胚胎”包括可移植胚胎以及代码为3和4的胚胎。胚胎回收期间采集的血样用于测量血清AMH、SAA和A/G比值。按AMH四分位数分组时，高AMH组和中高AMH组产生的总胚胎数量明显多于低AMH组。胚胎总数随着AMH水平升高而增加（r = 0.3336，P = 0.0009）。相关性分析揭示了AMH、α球蛋白和SAA之间的关联。此外，总胚胎与可移植胚胎之间（r = 0.6339，P < 0.0001）以及AMH与产量比值之间（r = 0.25583，P = 0.0119）观察到显著正相关。这些发现证实，AMH浓度是预测日本黑毛供体母牛产生的总胚胎和可移植胚胎数量的有价值的生殖标志物。
