Alvarez F, Bernard O, Homberg J C, Kreibich G
J Exp Med. 1985 May 1;161(5):1231-6. doi: 10.1084/jem.161.5.1231.
Children with autoimmune chronic active hepatitis may have high titers of antibodies detected by immunofluorescence staining of hepatocytes and tubular cells in rat liver and kidney sections, respectively. These antibodies are directed against antigens contained in microsomal fractions prepared from these two organs. We have found that sera from these patients recognized a 50,000 mol wt protein present in higher concentration in smooth microsome subfractions compared with rough microsome subfractions. This protein is an integral membrane protein and is not glycosylated. It is exposed on the cytoplasmic face of the endoplasmic reticulum and is rather resistant to proteolysis with proteinase K. Since patients with liver disease of different etiology and similar severity of cell lysis do not give rise to liver-kidney microsome antibody (LKMA), lysis of hepatocytes is apparently not a sufficient condition for their development.
自身免疫性慢性活动性肝炎患儿的抗体效价可能较高，分别通过大鼠肝脏和肾脏切片中肝细胞和肾小管细胞的免疫荧光染色检测到这些抗体。这些抗体针对的是从这两个器官制备的微粒体组分中所含的抗原。我们发现，这些患者的血清识别出一种50,000道尔顿分子量的蛋白质，该蛋白质在光滑微粒体亚组分中的浓度高于粗糙微粒体亚组分。这种蛋白质是一种整合膜蛋白，未被糖基化。它暴露在内质网的细胞质面上，对蛋白酶K的蛋白水解作用相当耐受。由于不同病因且细胞溶解严重程度相似的肝病患者不会产生肝肾微粒体抗体（LKMA），因此肝细胞溶解显然不是其产生的充分条件。
