Prince Riley, Roy Kai, Jesudason Nathan, Belinga Marc, Field Jacob, Heiesy Dylan, Arvidson Aaron, Menne Torrey, Selker John, Udell Chet
OPEnS Lab, Oregon State University, Corvallis, OR, United States.
HardwareX. 2024 Dec 30;21:e00617. doi: 10.1016/j.ohx.2024.e00617. eCollection 2025 Mar.
Environmental DNA (eDNA) is an ideal way of researching aquatic environments and determining what species are present in an area the biodiversity of an area, and if any invasive or endangered species are present. Traditional sampling of eDNA consists of manually filtering water, which is labor and cost-intensive for remote locations. Furthermore, commercialized solutions are either expensive or require a field operator to function. We have built a battery-powered eDNA sampler capable of autonomous multi-sampling for a greatly reduced price compared to existing technologies. Environmental DNA collection contains 3 main components: environmental DNA must be preserved, the filtered volume must be accurate, and there must be no cross-contamination between samples. The sampler operates in this way separating eDNA via filters, preserving DNA, and recording the filtered volume per sample. Our PolyWAG eDNA sampler system is a water sampling device that collects DNA samples via 47 mm filter and provides a non-invasive, safe and autonomous means of eDNA collection. The sampler can hold 24 filters and is designed to be easily replaced and reusable. A browser application is used for real-time monitoring, scheduling tasks, and data logging for time, pressure, flow, and filtered volume. Additionally, the sampler design is openly published, modular and is constantly being tested to help us optimize our software and hardware to give us the best results. The 13-step sampling sequence helps reduce cross contamination significantly. Our machine can be deployed for an extended period. It is completely autonomous and costs around $3800 for components or $6000 including labor.
环境DNA（eDNA）是研究水生环境、确定某一区域存在哪些物种、该区域生物多样性以及是否存在任何入侵物种或濒危物种的理想方法。传统的eDNA采样包括手动过滤水，对于偏远地区来说，这既耗费人力又成本高昂。此外，商业化解决方案要么昂贵，要么需要现场操作人员来操作。我们制造了一种电池供电的eDNA采样器，与现有技术相比，它能够以大幅降低的价格进行自主多次采样。环境DNA采集包含3个主要组成部分：环境DNA必须得到保存，过滤体积必须准确，并且样品之间不能有交叉污染。采样器通过过滤器分离eDNA、保存DNA并记录每个样品的过滤体积，以此方式运行。我们的PolyWAG eDNA采样器系统是一种水采样设备，它通过47毫米过滤器收集DNA样本，并提供一种非侵入性、安全且自主的eDNA采集方式。该采样器可容纳24个过滤器，设计易于更换且可重复使用。一个浏览器应用程序用于实时监测、安排任务以及记录时间、压力、流量和过滤体积的数据。此外，采样器的设计已公开，具有模块化特点，并且一直在进行测试，以帮助我们优化软件和硬件，从而获得最佳结果。13步采样序列有助于显著减少交叉污染。我们的机器可以长时间部署。它完全自主运行，组件成本约为3800美元，包括人工在内则为6000美元左右。
