毒素-抗毒素基因在变形链球菌于氮、脂肪酸或碳饥饿条件下培养时差异表达。
Toxin-antitoxin genes are differentially expressed in and mutans cultured under nitrogen, fatty acid, or carbon starvation conditions.
作者信息
Maciąg-Dorszyńska Monika, Olszewski Paweł, Karczewska Monika, Boss Lidia
机构信息
Department of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.
Department of Neuroscience, Uppsala University, Uppsala, Sweden.
出版信息
Front Microbiol. 2025 Jan 17;15:1528825. doi: 10.3389/fmicb.2024.1528825. eCollection 2024.
INTRODUCTION
The stringent response is one of the fundamental mechanisms that control and modulate bacterial adaptation to stress conditions, such as nutrient limitation. The accumulation of stringent response effectors, (p)ppGpp, causes differential expression of approximately 500 genes, including genes of bacterial endogenous toxin-antitoxin (TA) systems. However, the exact link between (p)ppGpp and toxin-antitoxin systems' activation, as well as toxin-antitoxin role in stress adaptation remains disputed.
METHODS
In this study, we performed a complex analysis of changes (RNA-Seq) in the toxin-antitoxin operons' transcription in response to nitrogen, fatty acid, or carbon starvation, in bacteria with different abilities of (p)ppGpp accumulation.
RESULTS AND DISCUSSION
Although we observed that in some cases (p)ppGpp accumulation appears to be crucial for transcriptional activation of TA genes (e.g., ), our data indicates that the general pattern of chromosomally encoded TA gene expression in differs depending on the nutrient distribution in the environment, regardless of the alarmone accumulation.
引言
严谨反应是控制和调节细菌适应应激条件（如营养限制）的基本机制之一。严谨反应效应物（p）ppGpp的积累会导致约500个基因的差异表达，包括细菌内源性毒素-抗毒素（TA）系统的基因。然而，（p）ppGpp与毒素-抗毒素系统激活之间的确切联系，以及毒素-抗毒素在应激适应中的作用仍存在争议。
方法
在本研究中，我们对具有不同（p）ppGpp积累能力的细菌中，毒素-抗毒素操纵子转录响应氮、脂肪酸或碳饥饿的变化（RNA测序）进行了综合分析。
结果与讨论
尽管我们观察到在某些情况下，（p）ppGpp的积累似乎对TA基因的转录激活至关重要（例如），但我们的数据表明，无论警报素的积累情况如何，不同环境中营养物质分布不同，染色体编码的TA基因表达的总体模式也不同。
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