Zhao Linna, Zhang Juanjuan, Song Qiuhang, Dai Cheng, Qin Yiping, Li Aiying
Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China.
Faculty of Nursing, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China.
Front Cell Dev Biol. 2025 Jan 17;12:1516898. doi: 10.3389/fcell.2024.1516898. eCollection 2024.
Heart failure (HF) is a prevalent cardiovascular disease that currently lacks effective treatment options due to its intricate pathogenesis. A recent study has linked disulfidoptosis, a novel form of cell demise, with the development of a range of diseases. Nonetheless, the effect of disulfidoptosis on the immune microenvironment of HF is not well comprehended. In this paper, bioinformatics analysis was performed to investigate how disulfidptosis-related genes (DRGs) affect the immune microenvironment of HF.
The expression of four DRGs was initially examined using bulk RNA-Seq and single-cell RNA sequencing data. A predictive model was subsequently developed. Consensus clustering was used to distinguish between the two clusters of DRGs. The effect of these DRGs on the characteristics of the immune microenvironment was further explored, such as infiltrating immune cells, immune response gene sets, and HLAs genes.
All four DRGs were dysregulated in HF samples. The predictive model based on these four DRGs effectively differentiated between HF patients and healthy individuals, which was validated in the experiment. These four DRGs were strongly associated with the abundance of infiltrating monocytes. Moreover, our analysis identified two distinct clusters of DRGs and these clusters exhibited differences in terms of immune cell abundance, immune response, and HLA gene expression. The biological functions associated with these differences were also revealed.
Our discovery underscores the pivotal role of DRGs in shaping the diversity and intricacy of the immune microenvironment in HF.
心力衰竭（HF）是一种常见的心血管疾病，由于其发病机制复杂，目前缺乏有效的治疗方法。最近的一项研究将二硫键介导的细胞死亡（一种新型细胞死亡形式）与一系列疾病的发生联系起来。然而，二硫键介导的细胞死亡对HF免疫微环境的影响尚未得到充分理解。本文进行了生物信息学分析，以研究二硫键介导的细胞死亡相关基因（DRGs）如何影响HF的免疫微环境。
首先使用批量RNA测序和单细胞RNA测序数据检测四个DRGs的表达。随后建立了一个预测模型。采用一致性聚类法区分DRGs的两个聚类。进一步探讨了这些DRGs对免疫微环境特征的影响，如免疫细胞浸润、免疫反应基因集和HLA基因。
在HF样本中，所有四个DRGs均表达失调。基于这四个DRGs的预测模型有效地区分了HF患者和健康个体，并在实验中得到验证。这四个DRGs与浸润单核细胞的丰度密切相关。此外，我们的分析确定了两个不同的DRGs聚类，这些聚类在免疫细胞丰度、免疫反应和HLA基因表达方面存在差异。还揭示了与这些差异相关的生物学功能。
我们的发现强调了DRGs在塑造HF免疫微环境的多样性和复杂性方面的关键作用。
