Ko Sydney, Mizumoto Kota
Graduate Program in Cell and Developmental Biology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.
Department of Zoology, Life Sciences Institute, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
MicroPubl Biol. 2025 Jan 18;2025. doi: 10.17912/micropub.biology.001447. eCollection 2025.
Green fluorescent proteins (GFPs) are invaluable tools for visualizing cells and proteins across model systems. Efforts have been made to generate brighter fluorescent proteins such as eGFP, GFPnovo2, mNeonGreen, and mStayGold. Here, we generated single-copy knock-in strains for these GFP variants and directly compared their brightness and photostability. We confirmed that mStayGold is brighter and more photostable than eGFP, GFPnovo2, and mNeonGreen, suggesting that it may hold advantages over other GFP variants in experiments where brightness and photostability are important factors.
绿色荧光蛋白（GFP）是用于跨模型系统可视化细胞和蛋白质的宝贵工具。人们已致力于生成更亮的荧光蛋白，如增强型绿色荧光蛋白（eGFP）、绿色荧光蛋白新变体2（GFPnovo2）、超亮绿色荧光蛋白（mNeonGreen）和持久金色荧光蛋白（mStayGold）。在此，我们为这些绿色荧光蛋白变体生成了单拷贝敲入菌株，并直接比较了它们的亮度和光稳定性。我们证实，持久金色荧光蛋白比增强型绿色荧光蛋白、绿色荧光蛋白新变体2和超亮绿色荧光蛋白更亮且光稳定性更高，这表明在亮度和光稳定性是重要因素的实验中，它可能比其他绿色荧光蛋白变体具有优势。
