Thi Thu Trinh Mai, Thoa Truong Huynh Kim, Thi Phuong Thao Dang
Laboratory of Molecular Biotechnology, University of Science-VNU.HCM, Viet Nam.
Department of Molecular and Environmental Biotechnology, Faculty of Biology -Biotechnology, University of Science-VNU.HCM, Viet Nam.
Toxicol Rep. 2025 Jan 8;14:101904. doi: 10.1016/j.toxrep.2025.101904. eCollection 2025 Jun.
Dimethyl sulfoxide (DMSO) is a polar aprotic solvent which is widely used in biological and medical studies and as a vehicle for pharmacological therapy. DMSO from 0.1 % to 0.5 %, particularly 0.3 % is commonly used as solvent to dissolve compounds when testing their effect on living cell, tissues including nerve cell. However, scientific data on the effects of DMSO on nervous system is limited. Here, we present our data of case study on investigation the effects of DMSO at 0.3 % concentration on nerve cell of model. We found that 0.3 % DMSO concentration had affected on the active zone and glutamate receptor. Notably, this study also revealed the synergistic effect of 0.3 % DMSO and loss function of dUCH (the homolog of Ubiquitin Carboxyl terminal Hydrolase -L1, UCH-L1 in ). This combination caused more serious abnormalities in synapse structure, particularly number of boutons on Neuromuscular Junction, NMJ. Furthermore, 0.3 % DMSO reduced the amount of ubiquitinylated protein aggregates in the indirect flight muscle of both normal and genectic defect fly model. Taken together, data in this sytudy indicated that 0.3 % DMSO caused the aberrant morphology of the synaptic structure and decreased the number of ubiquitinylated proteins in the indirect flight muscle of . The data from the study contributed new evidence of the effects of DMSO on the nervous system. Signigicantly, this study revealed that DMSO affected on neuron cell at low concentration which widely used as pharmacological solvent.
二甲基亚砜（DMSO）是一种极性非质子溶剂，广泛应用于生物学和医学研究以及作为药物治疗的载体。0.1%至0.5%，特别是0.3%的DMSO通常用作溶剂来溶解化合物，以测试它们对活细胞、包括神经细胞在内的组织的影响。然而，关于DMSO对神经系统影响的科学数据有限。在此，我们展示我们的案例研究数据，该研究调查了0.3%浓度的DMSO对模型神经细胞的影响。我们发现0.3%的DMSO浓度对活性区和谷氨酸受体有影响。值得注意的是，这项研究还揭示了0.3%的DMSO与dUCH（泛素羧基末端水解酶-L1，UCH-L1的同源物）功能丧失的协同作用。这种组合导致突触结构出现更严重的异常，特别是神经肌肉接头（NMJ）上的突触小体数量。此外，0.3%的DMSO减少了正常和基因缺陷果蝇模型间接飞行肌中泛素化蛋白聚集体的数量。综上所述，本研究中的数据表明，0.3%的DMSO导致突触结构形态异常，并减少了间接飞行肌中泛素化蛋白的数量。该研究的数据为DMSO对神经系统的影响提供了新的证据。重要的是，这项研究表明，DMSO在低浓度下会影响神经元细胞，而DMSO广泛用作药物溶剂。
