Muller de Moura Sarmento Silvia, Gomes Schmitt Elizandra, Smolski Dos Santos Laura, Erminda Schreiner Gênifer, Tamborena Malheiros Rafael, Klock Clóvis, Casanova Petry Charline, Gonçalves Itamar Luís, Manfredini Vanusa
Pós-Graduate Program in Biochemistry, Federal University of Pampa, BR 472, Km 585, Uruguaiana, RS, Brazil.
Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas, Universidade Federal do Pampa, Campus Uruguaiana, Rio Grande do Sul, Brazil.
Toxicol Rep. 2025 Jan 9;14:101901. doi: 10.1016/j.toxrep.2025.101901. eCollection 2025 Jun.
Vitamin D (VD) is shown to be a biochemical and physiological modulator of the body. Debates about route of administration, prescribed dosage and serum levels have arisen, and thus the interaction of VD with the body in overdose. Using an experimental model of Wistar rats of both sexes, rats were subdivided into 5 groups, which represents a control group, and 4 groups with VD treatments (2.500, 7.000, 14.000 and 21.000 IU/kg/week) for one month. Thereafter biochemical, hormonal, inflammatory and histological analyses were performed. Regarding the biochemical findings, there was an increase in the levels of the AST in comparison of the control group with the treatments with higher doses (14.000 IU and 21.000 IU). Furthermore, changes in the inflammatory cytokine profile were identified at doses of 14,000 IU and 21,000 IU, with an increase in inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) and a decrease in the anti-inflammatory IL-10. Histological evaluation of the liver tissue also revealed changes at the highest doses. Finally, in the evaluation of a murine physiological model, it showed that the supplementation of VD in overdoses there was an inflammatory exacerbation in the body, suggesting that the VD supplementation should be administered with caution, and takes into account physiological factors of the individual.
维生素D（VD）被证明是身体的一种生化和生理调节剂。关于给药途径、规定剂量和血清水平的争论已经出现，因此也有了VD过量时与身体的相互作用。使用雌雄Wistar大鼠的实验模型，将大鼠分为5组，其中一组为对照组，另外4组用VD进行处理（2500、7000、14000和21000国际单位/千克/周），持续一个月。此后进行了生化、激素、炎症和组织学分析。关于生化结果，与高剂量（14000国际单位和21000国际单位）处理组相比，对照组的谷草转氨酶水平有所升高。此外，在14000国际单位和21000国际单位的剂量下，炎症细胞因子谱发生了变化，炎症细胞因子（白细胞介素-1β、白细胞介素-6、白细胞介素-8和肿瘤坏死因子-α）增加，抗炎性白细胞介素-10减少。肝组织的组织学评估也显示在最高剂量时有变化。最后，在对小鼠生理模型的评估中，结果表明过量补充VD会使体内炎症加剧，这表明补充VD时应谨慎，并考虑个体的生理因素。
