Chen Zhuo, Shao Shunwei, Zhu Xi, Zhang Yu, Lan Zhendong, Jin Hui
Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, Zhanjiang, China.
Heliyon. 2025 Jan 10;11(2):e41903. doi: 10.1016/j.heliyon.2025.e41903. eCollection 2025 Jan 30.
Potato () is an important global food source, the growth of which can be severely impacted by bacterial infection. Despite extensive research, the molecular mechanisms of potato resistance to this pathogen are imperfectly known. Huashu No. 12, a tetraploid potato genotype, is highly resistant to . We inoculate Huashu No. 12 and Longshu No. 7 (highly susceptible to ) with to compare disease resistance in these two potato varieties. Huashu No. 12 has significantly higher resistance to infection than Longshu No. 7, with increased lignin content, and an abundance of callose and strong autofluorescence in the phloem sieve tube. Enzymes (e.g., superoxide dismutase, catalase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase) contribute to resistance in Huashu No. 12. Transcriptome sequencing reveals 659 differentially expressed genes between the two varieties, with the ethylene responsive factor family containing the most differentially expressed genes. Gene ontology and KEGG analyses provided further insights into the genetic basis and molecular mechanisms underlying plant defense against disease. By demonstrating the importance of enzymes and differential gene expression in Huashu No. 12 resistance to infection, the breeding of disease-resistant potato becomes increasingly feasible.
马铃薯（ ）是一种重要的全球粮食来源，其生长会受到细菌感染的严重影响。尽管进行了广泛研究，但马铃薯对这种病原体的抗性分子机制仍不完全清楚。四倍体马铃薯基因型华薯12号对 具有高度抗性。我们用 接种华薯12号和陇薯7号（对 高度敏感），以比较这两个马铃薯品种的抗病性。华薯12号对 感染的抗性明显高于陇薯7号，其木质素含量增加，韧皮部筛管中有丰富的胼胝质和强烈的自发荧光。酶（如超氧化物歧化酶、过氧化氢酶、过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶）对华薯12号的抗性有贡献。转录组测序揭示了两个品种之间有659个差异表达基因，其中乙烯响应因子家族的差异表达基因最多。基因本体论和KEGG分析为植物抵御 病害的遗传基础和分子机制提供了进一步的见解。通过证明酶和差异基因表达在华薯12号对 感染抗性中的重要性，抗病马铃薯的育种变得越来越可行。
