Grupa-Urbańska Anna, Szajko Katarzyna, Marczewski Waldemar, Lebecka Renata
Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, Młochów Division, Platanowa Str. 19, 05-831 Młochów, Poland.
Int J Mol Sci. 2025 Aug 28;26(17):8370. doi: 10.3390/ijms26178370.
causes soft rot in potato ( L.) tubers. We used bulk RNA-seq to compare the early transcriptional responses of the diploid F genotypes from the mapping population that varied in tuber resistance to . RNA was collected from wounded tubers inoculated with (B), wounded tubers treated with sterile water (W), and non-treated tubers (NT) at 8, 24, and 48 hours post-inoculation (hpi). The largest transcriptional divergence between resistant (R) and susceptible (S) genotypes occurred at 8 hpi, with R tubers showing stronger induction of phenylpropanoid biosynthesis, phenylalanine and tyrosine metabolism, amino sugar and nucleotide sugar metabolism, isoquinoline alkaloid biosynthesis, and glutathione metabolism. Phenylpropanoid biosynthesis was dominant in R tubers, in 17 differentially expressed genes (DEGs), consistent with rapid suberin and lignin deposition as a physical barrier. RT-qPCR of nine defence-related genes corroborated the RNA-seq trends. The suberisation-associated anionic peroxidase was located within a QTL for resistance on chromosome II, supporting its role as a candidate for future functional studies. This is the first transcriptome-based comparison of R and S potato genotypes challenged with , providing candidate pathways and genes that may guide future molecular breeding once their roles are validated.
导致马铃薯（L.）块茎软腐病。我们使用批量RNA测序来比较来自作图群体的二倍体F基因型在块茎对……抗性方面存在差异的早期转录反应。在接种后8小时、24小时和48小时，从接种了……的受伤块茎（B）、用无菌水处理的受伤块茎（W）和未处理的块茎（NT）中收集RNA。抗性（R）和感病（S）基因型之间最大的转录差异出现在接种后8小时，R块茎显示出苯丙烷生物合成、苯丙氨酸和酪氨酸代谢、氨基糖和核苷酸糖代谢、异喹啉生物碱生物合成以及谷胱甘肽代谢的更强诱导。苯丙烷生物合成在R块茎中占主导，有17个差异表达基因（DEG），这与作为物理屏障的木栓质和木质素的快速沉积一致。九个防御相关基因的RT-qPCR证实了RNA测序的趋势。与木栓化相关的阴离子过氧化物酶位于二号染色体上一个对……抗性的QTL内，支持其作为未来功能研究候选基因的作用。这是首次基于转录组对受……挑战的R和S马铃薯基因型进行比较，提供了一旦其作用得到验证可能指导未来分子育种的候选途径和基因。
