Cheng Li, Huang Zhenyang, He Jiawei, Zhang Xinyi, Di Jiangxue, Jiang Hanmei, Liu Yi
Hubei University of Traditional Chinese Medicine, Medicinal Plant Research and Development Center of Hubei Province, 430065, Hubei, China.
College of Management, Hubei University of Chinese Medicine, 16 West Road of Huangjiahu River, Wuhan, 430065, Hubei, China.
Heliyon. 2025 Jan 10;11(2):e41786. doi: 10.1016/j.heliyon.2025.e41786. eCollection 2025 Jan 30.
A growing number of studies have shown that hypertension symptoms are closely related to intestinal flora. The body's metabolites are closely related to disease states. Gouteng Granules (TG), a traditional Chinese medicine compound, has been proven to be an effective compound for the treatment of hypertension by traditional Chinese medicine diagnosis, but the target and therapeutic mechanism of TG on hypertension are still unclear.
We explored the mechanism of action of TG on hypertension by 16S rDNA gene sequencing and non-targeted metabolomics, verified the correlation between hypertension and intestinal flora, searched for potential markers of intestinal flora, and screened for the correlation between different flora and different metabolites, which facilitates a more scientific and reasonable guidance for the administration of TG.
The hypertensive model rats were induced by L-NAME. After drug administration, 16S rDNA gene sequencing and non-targeted metabolomics were applied to detect and analyze the intestinal flora and fecal metabolites of the rats in each group. The Spearman coefficient method was used to construct the interactions system of different flora and metabolites, which explore the potential mechanism of TG treatment hypertension.
After TG administration, the symptoms of hypertension were significantly reduced to normal in SD rats.16S rDNA gene sequencing and non-targeted metabolomics screened for differential flora , etc. and differential metabolites such as and . TG treatment of hypertension was found to be associated with vitamin B6 metabolic pathway and lipid metabolic pathway.
TG can treat hypertension by affecting differential strains and differential metabolites, providing a scientific basis for guiding the rational use of TG.
越来越多的研究表明，高血压症状与肠道菌群密切相关。机体代谢产物与疾病状态密切相关。钩藤颗粒（TG）作为一种中药复方，经中医诊断已被证明是治疗高血压的有效复方，但TG治疗高血压的靶点和作用机制仍不清楚。
通过16S rDNA基因测序和非靶向代谢组学方法探讨TG治疗高血压的作用机制，验证高血压与肠道菌群的相关性，寻找肠道菌群潜在标志物，筛选不同菌群与不同代谢产物之间的相关性，为TG的用药提供更科学合理的指导。
采用L-NAME诱导高血压模型大鼠。给药后，应用16S rDNA基因测序和非靶向代谢组学技术检测分析各组大鼠肠道菌群和粪便代谢产物。采用Spearman系数法构建不同菌群与代谢产物的相互作用体系，探讨TG治疗高血压的潜在机制。
给予TG后，SD大鼠高血压症状明显减轻至正常。16S rDNA基因测序和非靶向代谢组学筛选出差异菌群等以及差异代谢产物如……和……。发现TG治疗高血压与维生素B6代谢途径和脂质代谢途径有关。
TG可通过影响差异菌株和差异代谢产物治疗高血压，为指导TG的合理使用提供科学依据。
