Doshi Pratik, Klas Matej, Kyzek Stanislav, Zahoranová Anna, Šerá Božena
Department of Environmental Ecology and Landscape Management, Faculty of Natural Sciences, Comenius University Bratislava, Ilkovičova 6, 84215, Bratislava, Slovak Republic.
Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava, Mlynská Dolina, 84248, Bratislava, Slovak Republic.
Heliyon. 2025 Jan 16;11(2):e42038. doi: 10.1016/j.heliyon.2025.e42038. eCollection 2025 Jan 30.
Entomopathogenic nematodes are currently being tested for their efficiency in controlling several insect pests. In recent years, non-thermal plasma has been investigated as a state-of-the-art technology for its disinfection/decontamination properties on the seed surface. In addition, it is also used to induce seed germination. In this investigation, the effect of plasma activated water (PAW) was tested on three EPN species, namely Filipjev (1934), Weiser (1955), and Poinar (1976). Seven different PAW prepared at different treatment times, that is, (1s, 3s, 5s, 10s, 20s, 60s, 90s) were tested directly on the three selected nematode species. Distilled water was used as a control treatment (0s). In the case of , significantly higher mortality was observed in PAW preparation times of 5, 10, 20, 60 and 90s compared to the control. In the case of , significantly high mortality was observed for PAW preparation times of 10, 20, 60 and 90s. However, was found to have the least sensitivity against all PAW treatments, with a maximum mortality of 14 % (<20 %), indicating the potential synergy between PAW and EPNs. The possibility of combined treatments in the context of integrated pest management is presented and discussed.
目前正在测试昆虫病原线虫对几种害虫的防治效果。近年来，非热等离子体作为一种先进技术，因其对种子表面的消毒/去污特性而受到研究。此外，它还被用于诱导种子萌发。在本研究中，测试了等离子体活化水（PAW）对三种昆虫病原线虫的影响，即 Filipjev（1934）、Weiser（1955）和 Poinar（1976）。对在不同处理时间制备的七种不同PAW（即1秒、3秒、5秒、10秒、20秒、60秒、90秒）直接测试三种选定的线虫物种。蒸馏水用作对照处理（0秒）。在[某种线虫名称]的情况下，与对照相比，在PAW制备时间为5秒、10秒、20秒、60秒和90秒时观察到显著更高的死亡率。在[另一种线虫名称]的情况下，在PAW制备时间为10秒、20秒、60秒和90秒时观察到显著高的死亡率。然而，[第三种线虫名称]被发现对所有PAW处理的敏感性最低，最大死亡率为14%（<20%），表明PAW与昆虫病原线虫之间存在潜在的协同作用。本文提出并讨论了在综合虫害管理背景下联合处理的可能性。
