Plasma Research Group, School of Food Science and Environmental Health, Technological University of Dublin, Dublin, Ireland.
School of Chemical and Biomolecular Engineering, University of Sydney, Sydney, Australia.
Appl Environ Microbiol. 2020 Apr 17;86(9). doi: 10.1128/AEM.02619-19.
Atmospheric cold plasma (ACP) treatment is an emerging food technology for product safety and quality retention, shelf-life extension, and sustainable processing. The activated chemical species of ACP can act rapidly against microorganisms without leaving chemical residues on food surfaces. The main objectives of this study were to investigate the efficiency and mechanisms of inactivation of fungal spores and biofilms by ACP and to understand the effects of the gas-mediated and liquid-mediated modes of application against important fungal contaminants. was selected as the model microorganism. spores were exposed to either gas plasma (GP) or plasma-activated water (PAW), whereas gas plasma alone was used to treat biofilms. This study demonstrated that both GP and PAW treatments independently resulted in significant decreases of metabolic activity and spore counts, with maximal reductions of 2.2 and 0.6 log units for GP and PAW, respectively. The characterization of the reactive oxygen and nitrogen species in PAW and spore suspensions indicated that the concentration of secondary reactive species was an important factor influencing the antimicrobial activity of the treatment. The biofilm study showed that GP had detrimental effects on biofilm structure; however, the initial inoculum concentration prior to biofilm formation can be a crucial factor influencing the fungicidal effects of ACP. The production of mycotoxin-free food remains a challenge in both human and animal food chains. , a mycotoxin-producing contaminant of economically important crops, was selected as the model microorganism to investigate the efficiency and mechanisms of ACP technology against fungal contaminants of food. Our study directly compares the antifungal properties of gas plasma (GP) and plasma-activated water (PAW) against fungi as well as reporting the effects of ACP treatment on biofilms produced by .
大气压冷等离子体（ACP）处理是一种新兴的食品技术，可用于保障食品安全和保留产品质量、延长货架期和实现可持续加工。ACP 的激活化学物质可以快速作用于微生物，而不会在食品表面留下化学残留物。本研究的主要目的是研究 ACP 对真菌孢子和生物膜的灭活效率和机制，并了解气体介导和液体介导应用模式对重要真菌污染物的影响。 被选为模型微生物。 孢子分别暴露于气体等离子体（GP）或等离子体激活水（PAW）中，而单独使用气体等离子体处理 生物膜。本研究表明，GP 和 PAW 处理均可独立导致 代谢活性和孢子计数显著下降，GP 和 PAW 的最大减少量分别为 2.2 和 0.6 个对数单位。对 PAW 和孢子悬浮液中活性氧和氮物种的表征表明，二次活性物种的浓度是影响处理抗菌活性的重要因素。生物膜研究表明，GP 对生物膜结构有不利影响；然而，生物膜形成前的初始接种浓度可能是影响 ACP 杀菌效果的关键因素。在人类和动物食物链中，生产无真菌毒素的食品仍然是一个挑战。 ，一种经济上重要作物的真菌毒素产生污染物，被选为模型微生物，以研究 ACP 技术对真菌污染物的效率和机制。我们的研究直接比较了气体等离子体（GP）和等离子体激活水（PAW）对真菌的抗真菌特性，并报告了 ACP 处理对 生物膜的影响。
