Zhang Tian-Tian, Lu Chuan-Li, Jiang Jian-Guo
College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.
Food Funct. 2014 Aug;5(8):1747-54. doi: 10.1039/c4fo00169a.
In this work, a phytochemical investigation was conducted on Amomum tsaoko Crevost et Lemaire, a traditional Chinese spice. Based on spectroscopic methods including MS, (1)H-NMR, (13)C-NMR, DEPT135 and HMQC spectroscopy, eight main chemical compositions, sitosterol, daucosterol, meso-hannokinol, quercetin, epicatechin, quercetin-7-O-β-glucoside, quercetin-3-O-β-glucoside, and catechol, were isolated and identified from A. tsaoko, among which quercetin, quercetin-7-O-β-glucoside and quercetin-3-O-β-glucoside were first found in A. tsaoko. Their bioactivities were evaluated by the inhibitory effect on NO production in LPS-stimulated macrophage RAW 264.7 cells, the protective effect on H2O2-induced apoptosis of PC-12 cells and the DPPH radical scavenging assay. Epicatechin exhibited excellent anti-inflammatory properties; its inhibition rate was up to 63.65% at a concentration of 100 μg mL(-1). In addition, quercetin showed the strongest neuroprotective effect of PC-12 cells induced by H2O2, and the cell viability was up to 78.9% at a concentration of 50 μg mL(-1). Quercetin also exhibited excellent DPPH radical-scavenging activity at a concentration of 100 μg mL(-1) (DPPH radical inhibition rate > 80%), which was very close to Vc at the same concentration. It is concluded that, in A. tsaoko, different ingredients have their own health functions.
本研究对中国传统香料草果（Amomum tsaoko Crevost et Lemaire）进行了植物化学研究。基于质谱（MS）、氢核磁共振（¹H-NMR）、碳核磁共振（¹³C-NMR）、DEPT135和HMQC光谱等方法，从草果中分离并鉴定出8种主要化学成分，即甾醇、胡萝卜苷、中汉诺醇、槲皮素、表儿茶素、槲皮素-7-O-β-葡萄糖苷、槲皮素-3-O-β-葡萄糖苷和儿茶酚，其中槲皮素、槲皮素-7-O-β-葡萄糖苷和槲皮素-3-O-β-葡萄糖苷首次在草果中发现。通过检测其对脂多糖（LPS）刺激的巨噬细胞RAW 264.7细胞中一氧化氮（NO）生成的抑制作用、对过氧化氢（H₂O₂）诱导的PC-12细胞凋亡的保护作用以及二苯基苦味酰基自由基（DPPH）清除试验，对它们的生物活性进行了评估。表儿茶素表现出优异的抗炎特性；在浓度为100 μg mL⁻¹时，其抑制率高达63.65%。此外，槲皮素对H₂O₂诱导的PC-12细胞显示出最强的神经保护作用，在浓度为50 μg mL⁻¹时，细胞活力高达78.9%。槲皮素在浓度为100 μg mL⁻¹时还表现出优异的DPPH自由基清除活性（DPPH自由基抑制率>80%），这与相同浓度下的维生素C非常接近。研究得出结论，在草果中，不同成分具有各自的保健功能。
