Meibuko Ngakwi Masai, Mtui Hosea Dunstan, Baltazari Anna
Tanzania Agriculture Research Institute, Tumbi Centre, Tabora, Tanzania.
Department of Crop Science and Horticulture, College of Agriculture, Sokoine University of Agriculture, Chuo-Kikuu, Morogoro, Tanzania.
Front Plant Sci. 2025 Jan 17;15:1453538. doi: 10.3389/fpls.2024.1453538. eCollection 2024.
A drawback associated with conventional clonal propagation of cassava is its limited multiplication ratio, which poses challenges for both cassava crop enhancement and seed system initiatives. This study was aimed to evaluate the response of varieties on the sprouting ability of their leaf buds from the upper, middle, and lower positions of mature plant branches for rapid multiplication of planting materials. Five varieties, namely, Kizimbani, Mkombozi, Mkumba, TARICASS 4, and Mkuranga 1, were selected. The criteria for selection were resistance to green mites (), a common cassava serious insect pest; Cassava Mosaic Disease (CMD) and Cassava Brown Streak Disease (CBSD), the most serious cassava viral diseases; and high-yielding, dry matter, and starch content potentials. The experiment was conducted in a screen house where the leaf buds from mature mother plant branches were planted in a growth cage measuring 3.0 m × 1.5 m × 3.5 m made of metal pipes and covered with a transparent polyethylene sheet for sprouting. The design used was randomized complete block design (RCBD) in a split-split plot arrangement with three replications. Varieties were the main plot factor, media as a subplot factor, and leaf bud position as a sub-sub plot factor. Results showed that variety had significant effects (p < 0.001) on number of nodes, percent sprouting, number of sprouts, and days to 50% sprouting, and a significant difference (p < 0.01) on percent survival rates and days to first sprouting. The effects of leaf bud position had significant effects (p < 0.001) on the number of leaf buds, percent sprouting, number of sprouts, days to first sprouting, and percent survival rates. The media used showed significant effects on days to first sprouting (p < 0.01) and first sprouting. TARICASS 4 was the most promising variety for rapid multiplication of cassava planting materials using the leaf bud technique due to its superior performance. The use of coco peat and peat moss media generally led to early and more successful sprouting. These results are important as a basis for selecting varieties for propagation using the leaf bud technique for rapid multiplication of planting materials for breeding and seed production purposes.
木薯传统克隆繁殖的一个缺点是其繁殖系数有限,这给木薯作物改良和种子系统计划都带来了挑战。本研究旨在评估不同品种成熟植株枝条上、中、下部位叶芽的发芽能力,以实现种植材料的快速繁殖。选取了五个品种,即基齐姆巴尼、姆科博齐、姆昆巴、TARICASS 4和姆库兰加1号。选择标准包括对绿螨(一种常见且严重的木薯害虫)、木薯花叶病(CMD)和木薯褐色条纹病(CBSD,最严重的木薯病毒病)具有抗性,以及具有高产、高干物质和高淀粉含量的潜力。实验在温室中进行,将成熟母株枝条上的叶芽种植在一个由金属管制成、尺寸为3.0米×1.5米×3.5米的生长笼中,并用透明聚乙烯薄膜覆盖以促进发芽。采用的设计是随机完全区组设计(RCBD),裂区裂区排列,重复三次。品种为主区因素,培养基为副区因素,叶芽位置为副副区因素。结果表明,品种对节点数、发芽率、芽数和50%发芽天数有显著影响(p < 0.001),对成活率和首次发芽天数有显著差异(p < 0.01)。叶芽位置对叶芽数、发芽率、芽数、首次发芽天数和成活率有显著影响(p < 0.001)。所用培养基对首次发芽天数(p < 0.01)和首次发芽有显著影响。由于其优异的表现,TARICASS 4是使用叶芽技术快速繁殖木薯种植材料最有前景的品种。使用椰糠和泥炭藓培养基通常能实现更早且更成功的发芽。这些结果对于选择利用叶芽技术进行繁殖的品种,以便为育种和种子生产目的快速繁殖种植材料具有重要意义。