Peng Wei, Zhao Yali, Yang Ningning, Fang Yan, Wu Yintong, Feng Zhenzhong, Wu Qiang, Wang Xian
Department of Pathology, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.
Department of Pathology, School of Basic Medical Science, Anhui Medical University, Hefei, China.
Discov Oncol. 2025 Feb 3;16(1):113. doi: 10.1007/s12672-025-01843-6.
FCER1G as an immune-associated protein, which belongs to the immunoglobulin superfamily and is involved in mediating and executing antibody-mediated immune responses. However, the role of FCER1G in cancers remains controversial. Our objectives were to study the association between FCER1G and tumor- infiltrating immune cells (TIICs) as well as the predictive significance of FCER1G.
The expression of FCER1G and its prognostic value in ESCC was examined by The Cancer Genome Atlas and Gene Expression Omnibus databases. We also evaluated the relationship between FCER1G expression and 22 TIICs. Immunohistochemistry was used to detect the expression and distribution of FCER1G. Double immunofluorescence was used to detect the co-expression of FCER1G and CD163 positive cells. Kaplan-Meier survival curves and Cox regression analysis was performed to determine the prognostic significance of FCER1G and CD163.
The analysis revealed that FCER1G was upregulated in ESCC, which was distributed more in the intra-tumor mesenchyme than in the cancer nests. The more infiltration in intra-tumor mesenchyme the worse the overall survival (OS) for patients with ESCC. The infiltration of FCER1G cells was positively correlated with that of M2 macrophages and most of the CD163 M2 macrophages expressed FCER1G. The more the infiltration of FCER1G M2 macrophages, the worse the OS of ESCC patients. FCER1G and TNM stage were identified as independent risk factors affecting the OS of ESCC patients.
FCER1G cells infiltration may help to predict the prognosis of ESCC. The combined detection of FCER1G and CD163 has a higher prognostic value.
FCER1G作为一种免疫相关蛋白，属于免疫球蛋白超家族，参与介导和执行抗体介导的免疫反应。然而，FCER1G在癌症中的作用仍存在争议。我们的目的是研究FCER1G与肿瘤浸润免疫细胞（TIICs）之间的关联以及FCER1G的预测意义。
通过癌症基因组图谱和基因表达综合数据库检测FCER1G在食管鳞状细胞癌（ESCC）中的表达及其预后价值。我们还评估了FCER1G表达与22种TIICs之间的关系。采用免疫组织化学法检测FCER1G的表达和分布。采用双重免疫荧光法检测FCER1G与CD163阳性细胞的共表达。进行Kaplan-Meier生存曲线和Cox回归分析，以确定FCER1G和CD163的预后意义。
分析显示，FCER1G在ESCC中上调，在肿瘤内间质中的分布多于癌巢。肿瘤内间质中浸润越多，ESCC患者的总生存期（OS）越差。FCER1G细胞的浸润与M2巨噬细胞的浸润呈正相关，大多数CD163 M2巨噬细胞表达FCER1G。FCER1G M2巨噬细胞浸润越多，ESCC患者的OS越差。FCER1G和TNM分期被确定为影响ESCC患者OS的独立危险因素。
FCER1G细胞浸润可能有助于预测ESCC的预后。联合检测FCER1G和CD163具有更高的预后价值。
