Liu Haifeng, Li Pinhan, Xin Jialiang, Huang Haocheng, Yang Yuxue, Deng Hongchuan, Zhou Ziyao, Zhong Zhijun, Peng Guangneng, Chen Dechun, He Changliang
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, China.
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China.
Probiotics Antimicrob Proteins. 2025 Feb 3. doi: 10.1007/s12602-025-10473-x.
Milk is a rich source of probiotics, particularly lactic acid bacteria (LAB), which have been shown to promote gut health, support the immune system, enhance digestion, and prevent pathogen colonization. This study aimed to isolate and identify LAB strains from giant panda (Ailuropoda melanoleuca) milk, evaluate their probiotic properties, and analyze the genomic characteristics of a promising strain. Thirteen LAB strains were isolated from 12 samples of giant panda milk. Among all LAB strains, Lactiplantibacillus plantarum PM8 (PM8) demonstrated probiotic properties and safety features. It exhibited strong growth performance, high antipathogenic activity against four pathogens, and strong survival rates under simulated gastrointestinal conditions. PM8 also showed excellent adhesion capabilities to Caco-2 cells. Additionally, safety assessment revealed no hemolysin production and minimal antibiotic resistance, making it a promising candidate for probiotic applications. The genome of PM8 consists of 3,227,035 bp with a GC content of 44.60% and contains 3171 coding sequences, including 113 carbohydrate-active enzyme genes and genes related to exopolysaccharides synthesis, vitamin B biosynthesis, adhesion, antioxidant activity, and bile salt hydrolysis. Notably, it contains genes involved in nonribosomally synthesized secondary metabolite and bacteriocin production. The genomic safety analysis confirmed that PM8 lacks the capacity to transmit bacterial antimicrobial resistance and is non-pathogenic to both humans and animals. These findings suggest that PM8 holds considerable potential for enhancing gut health and supporting the development of safe probiotic products.
牛奶是益生菌的丰富来源，尤其是乳酸菌（LAB），已证明其能促进肠道健康、支持免疫系统、增强消化功能并防止病原体定植。本研究旨在从大熊猫（Ailuropoda melanoleuca）乳汁中分离和鉴定乳酸菌菌株，评估其益生菌特性，并分析一株有潜力菌株的基因组特征。从12份大熊猫乳汁样本中分离出13株乳酸菌菌株。在所有乳酸菌菌株中，植物乳杆菌PM8（PM8）表现出益生菌特性和安全特性。它具有强大的生长性能，对四种病原体具有高抗病原活性，并且在模拟胃肠道条件下具有较高的存活率。PM8对Caco-2细胞也表现出优异的黏附能力。此外，安全性评估显示无溶血素产生且抗生素耐药性极低，使其成为益生菌应用的有潜力候选菌株。PM8的基因组由3,227,035 bp组成，GC含量为44.60%，包含3171个编码序列，包括113个碳水化合物活性酶基因以及与胞外多糖合成、维生素B生物合成、黏附、抗氧化活性和胆盐水解相关的基因。值得注意的是，它包含参与非核糖体合成的次级代谢产物和细菌素产生的基因。基因组安全性分析证实PM8缺乏传播细菌抗菌耐药性的能力，并且对人和动物均无致病性。这些发现表明PM8在促进肠道健康和支持安全益生菌产品开发方面具有相当大的潜力。
