Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Chengdu Wildlife Institute, Chengdu Zoo, Chengdu, China.
Probiotics Antimicrob Proteins. 2019 Jun;11(2):535-544. doi: 10.1007/s12602-018-9402-2.
Weissella confusa has recently received attention for its probiotic potential. Some W. confusa and Weissella cibaria strains isolated from fermented foods show favorable probiotic effects. However, the probiotic properties of W. confusa isolated from giant panda remain unreported to date. Thus, this study isolated a W. confusa strain from giant panda feces and then investigated its characteristics and probiotic properties. A lactic acid bacteria strain was isolated from giant panda fecal samples. The isolated strain was screened by in vitro probiotic property tests, including in vitro antimicrobial test, antioxidant test, surface hydrophobicity, and stress resistance. On the basis of biochemical identification and 16S rDNA sequencing, the W. confusa strain was identified as BSP201703. This Weissella confusa strain can survive at pH 2 and 0.3% (w/v) concentration of bile salt environment and inhibit common intestinal pathogens. It also possesses an in vitro antioxidant capacity, a high auto-aggregation ability, and a high surface hydrophobicity. BSP201703 might serve as a probiotic to giant pandas.
混乱魏斯氏菌最近因其益生菌潜力而受到关注。一些从发酵食品中分离出的 W. confusa 和 Weissella cibaria 菌株显示出良好的益生菌作用。然而,从大熊猫粪便中分离出的 W. confusa 的益生菌特性迄今尚未报道。因此,本研究从大熊猫粪便中分离出一株混乱魏斯氏菌,并对其特性和益生菌特性进行了研究。从大熊猫粪便样本中分离出一株乳酸菌。通过体外益生菌特性试验,包括体外抗菌试验、抗氧化试验、表面疏水性和应激抗性,对分离株进行了筛选。基于生化鉴定和 16S rDNA 测序,将 W. confusa 菌株鉴定为 BSP201703。该混乱魏斯氏菌株能在 pH 2 和 0.3%(w/v)胆盐浓度的环境中存活,并能抑制常见的肠道病原体。它还具有体外抗氧化能力、高自聚集能力和高表面疏水性。BSP201703 可能成为大熊猫的益生菌。