Zhang Yaoyi, Lv Shuai, Huang Pinyuan, Xiao Lingmin, Lin Nan, Huang En
Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
Department of Pediatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China.
NPJ Sci Food. 2025 Feb 4;9(1):16. doi: 10.1038/s41538-025-00378-y.
Research indicated that berberine (BBR) plays a protective role in modulating Alzheimer's disease (AD). This study aimed to explore the target genes of BBR associated with AD therapy using a network pharmacology study. Through network pharmacology analysis, two main potential target genes, β-amyloid precursor protein (APP) and peroxisome proliferator-activated receptor gamma (PPARG), of BBR for AD therapy were screened out. Further experiments demonstrated that BV2 and C8-D1A treated with BBR were decreased in the mRNA and protein expression of APP and presenilin 1 while PPARG was increased with a reduction in the NF-κB pathway. A similar result was shown in vivo. Through a network pharmacology study, this study supported that BBR played a protective role in the AD mice model via blocking APP processing and amyloid plaque formation. It also promotes PPARG expression to blockage of NF-κB pathway-mediated inflammatory response and neuroinflammation.
研究表明，黄连素（BBR）在调节阿尔茨海默病（AD）方面发挥着保护作用。本研究旨在通过网络药理学研究探索与AD治疗相关的BBR靶基因。通过网络药理学分析，筛选出BBR治疗AD的两个主要潜在靶基因，即β-淀粉样前体蛋白（APP）和过氧化物酶体增殖物激活受体γ（PPARG）。进一步实验表明，用BBR处理的BV2和C8-D1A中，APP和早老素1的mRNA和蛋白表达降低，而PPARG增加，同时NF-κB途径减少。体内实验也显示了类似结果。通过网络药理学研究，本研究支持BBR通过阻断APP加工和淀粉样斑块形成在AD小鼠模型中发挥保护作用。它还通过促进PPARG表达来阻断NF-κB途径介导的炎症反应和神经炎症。
