Zhang Jia, Yang Dan, Zeng Yafei, Guo Kaini, Zhang Jiajian, Huang Yan, Sui Yuzhen, Liu Qimin, Mo Xiaoxuan, Zhao Chenxu, Wang Jianguo
College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Anim Biosci. 2025 May;38(5):981-992. doi: 10.5713/ab.24.0567. Epub 2025 Jan 24.
This study aimed to explore novel aspects of disease prevention and control in Guanzhong dairy goats through the application of metabolomics and lipidomics.
In this study, plasma samples were collected from 96 primiparous Guanzhong dairy goats with similar body condition scores (2.75±0.15, mean±standard deviation) on the day of calving. The aim was to identify key differences in metabolite expression between diseased and healthy animals using metabolomics and lipidomics.
Twenty-three differential metabolites and 30 differentially altered lipids were identified, which were associated with various metabolic pathways, including phenylalanine and tyrosine metabolism, aminoacyl-tRNA biosynthesis, and glycerophospholipid metabolism. Our research revealed significant differences in the regulation of calcium-related hormones and associated metabolites between subclinical hypocalcemic and healthy dairy goats. Specifically, parathyroid hormone and aspartate aminotransferase were positively correlated in the healthy group and negatively correlated in the subclinical hypocalcemic group.
The identification of phenylalanine and phosphatidylserine as potential biomarkers for subclinical hypocalcemia in dairy goats offers a novel approach to managing this condition, potentially transforming prevention and control strategies in the dairy goat industry.
本研究旨在通过代谢组学和脂质组学的应用，探索关中奶山羊疾病预防与控制的新方面。
在本研究中，于产犊当天从96只体况评分相似（2.75±0.15，均值±标准差）的初产关中奶山羊采集血浆样本。目的是利用代谢组学和脂质组学确定患病动物与健康动物之间代谢物表达的关键差异。
鉴定出23种差异代谢物和30种差异改变的脂质，它们与多种代谢途径相关，包括苯丙氨酸和酪氨酸代谢、氨酰 - tRNA生物合成以及甘油磷脂代谢。我们的研究揭示了亚临床低钙血症奶山羊与健康奶山羊在钙相关激素及相关代谢物调节方面存在显著差异。具体而言，甲状旁腺激素与天冬氨酸转氨酶在健康组中呈正相关，而在亚临床低钙血症组中呈负相关。
将苯丙氨酸和磷脂酰丝氨酸鉴定为奶山羊亚临床低钙血症的潜在生物标志物，为管理这种病症提供了一种新方法，可能会改变奶山羊产业的预防和控制策略。
