Hou Tianze, Zheng Baoqiang, Peng Fucheng, Jiang Zehui, Zhang Wenbo, Wang Yan
Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing, China.
Front Plant Sci. 2025 Jan 20;15:1505199. doi: 10.3389/fpls.2024.1505199. eCollection 2024.
Long-term space missions will require high-quality plants that are edible, medicinal, and ornamental, to support the physical and mental health of astronauts under altered gravity conditions. Anthocyanins play a key role in enhancing the medicinal and edible value and ornamental properties of plants. However, under simulated microgravity, the transcription control of anthocyanin biosynthesis is not clear. Here, in order to investigate the influences of simulated microgravity on the anthocyanin accumulation further, clones of were exposed for 20 days to simulated microgravity conditions. The anthocyanin content in leaves increased in the simulated microgravity conditions compared with that in gravity-treated clones. Furthermore, based on the transcriptome sequencing, differentially expressed genes (DEGs), and weighted gene co-expression network analysis combined with RT-qPCR, we identified one WRKY gene, , from a under simulated microgravity conditions, which indicated that may be involved in anthocyanin biosynthesis under simulated microgravity conditions. A more in-depth analysis evaluating the function of , transcription factor gene , was silenced by virus-induced gene silencing under gravity conditions, which resulted in the increase of anthocyanin accumulation in leaves, and the expression levels of anthocyanin biosynthesis pathway (ABP) structural genes, including , , , , and were increased significantly. This research provides new insights into how altered gravity can affect anthocyanin synthesis in plants and illuminated the regulatory effects of on the leaves' pigmentation and anthocyanin biosynthesis in under gravity and simulated microgravity.
长期太空任务将需要高品质的可食用、药用和观赏性植物，以在重力改变的条件下支持宇航员的身心健康。花青素在提高植物的药用和食用价值以及观赏特性方面起着关键作用。然而，在模拟微重力条件下，花青素生物合成的转录调控尚不清楚。在此，为了进一步研究模拟微重力对花青素积累的影响，将[植物名称]的克隆体暴露于模拟微重力条件下20天。与重力处理的克隆体相比，模拟微重力条件下[植物名称]叶片中的花青素含量增加。此外，基于转录组测序、差异表达基因（DEGs）以及加权基因共表达网络分析并结合RT-qPCR，我们在模拟微重力条件下从[植物名称]中鉴定出一个WRKY基因，[基因名称]，这表明[基因名称]可能参与模拟微重力条件下的花青素生物合成。对转录因子基因[基因名称]功能进行更深入分析时，在重力条件下通过病毒诱导基因沉默使其沉默，这导致叶片中花青素积累增加，并且花青素生物合成途径（ABP）结构基因的表达水平，包括[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5]显著增加。本研究为重力改变如何影响植物中花青素合成提供了新见解，并阐明了[基因名称]在重力和模拟微重力条件下对[植物名称]叶片色素沉着和花青素生物合成的调控作用。
