Yuan Baoqi, Li Chuang, Wang Qingfeng, Yao Qi, Guo Xiaowei, Zhang Yuhang, Wang Zhongwei
Institute of Economic Plants, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun, China.
Front Plant Sci. 2025 Jan 20;15:1515060. doi: 10.3389/fpls.2024.1515060. eCollection 2024.
Potato ( L.), as the world's fourth largest food crop, plays a crucial role in ensuring food security through its disease resistance. The gene family is known to play a pivotal role in plant disease resistance responses; however, its specific functions in potato remain unclear. In this study, we conducted the first comprehensive identification and analysis of 28 gene family members in potato, examining their gene structures, chromosomal locations, expression patterns, and functional characteristics. Gene structure analysis revealed that most members contain the typical CC-NBS-LRR domains, with exon numbers ranging from 1 to 6. Phylogenetic analysis grouped these genes into four evolutionary clades, indicating a high level of conservation. Cis-regulatory element analysis identified that the promoter region of is enriched with pathogen-responsive elements such as the WUN-motif and MYC, suggesting its potential role in disease defense. Expression pattern analysis showed that , , and are highly expressed in various tissues, indicating their involvement in basic physiological functions, whereas and are mainly induced under specific pathogen infection conditions. Transcriptome and qRT-PCR analyses further revealed functional divergence of the gene family in response to potato scab disease. Notably, was significantly downregulated in both resistant and susceptible cultivars, suggesting its crucial role in the early stages of pathogen recognition. Subcellular localization experiments showed that the StRPP13-11 protein is localized in the chloroplast. Combined with transcriptome-based functional enrichment analysis, this finding implies that StRPP13-11 may participate in disease defense by regulating photosynthesis-related genes and the dynamic balance of reactive oxygen species within the chloroplast. This study provides new insights into the potential functions of the gene family in potato disease resistance mechanisms, offering valuable genetic resources and theoretical support for future disease-resistant breeding programs.
马铃薯（Solanum tuberosum L.）作为世界第四大粮食作物，通过其抗病性在确保粮食安全方面发挥着关键作用。已知RPP13基因家族在植物抗病反应中起关键作用；然而，其在马铃薯中的具体功能仍不清楚。在本研究中，我们首次对马铃薯中28个RPP13基因家族成员进行了全面鉴定和分析，研究了它们的基因结构、染色体定位、表达模式和功能特性。基因结构分析表明，大多数成员含有典型的CC-NBS-LRR结构域，外显子数量从1到6不等。系统发育分析将这些基因分为四个进化分支，表明其具有高度保守性。顺式调控元件分析确定，RPP13的启动子区域富含WUN-motif和MYC等病原体响应元件，表明其在病害防御中的潜在作用。表达模式分析表明，StRPP13-1、StRPP13-2和StRPP13-3在各种组织中高度表达，表明它们参与基本生理功能，而StRPP13-4和StRPP13-5主要在特定病原体感染条件下被诱导。转录组和qRT-PCR分析进一步揭示了RPP13基因家族在应对马铃薯疮痂病时的功能差异。值得注意的是，StRPP13-11在抗病和感病品种中均显著下调，表明其在病原体识别早期阶段的关键作用。亚细胞定位实验表明，StRPP13-11蛋白定位于叶绿体。结合基于转录组的功能富集分析，这一发现意味着StRPP13-11可能通过调节叶绿体中与光合作用相关的基因和活性氧的动态平衡来参与病害防御。本研究为RPP13基因家族在马铃薯抗病机制中的潜在功能提供了新的见解，为未来的抗病育种计划提供了有价值的遗传资源和理论支持。
