Domínguez Mario, de Marcos Susana, Galbán Javier
Analytical Chemistry Department, University of Zaragoza, 50009, Saragossa, Spain.
Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, 50009, Saragossa, Spain.
Mikrochim Acta. 2025 Feb 4;192(3):132. doi: 10.1007/s00604-025-06964-x.
The without-seeds in situ formation of gold nanoparticles from NADPH and its application to the colorimetric determination of atropine (a tropane alkaloid) in cereals is reported. The method is based on a chemical hydrolysis, followed by an enzymatic oxidation by NADP catalyzed by tropinone reductase in the presence of Au(III) or Au(III)/Pt(II). During this reaction, the formed NADPH reduces the metal ion precursor to AuNPs (or AuPtNPs) and the absorption due to the plasmon band (550 nm or 575 nm) is measured. The method (AuPtNPs) allows the determination of the analyte in the concentration range 0.025 to 0.09 mM with an RSD of 3% (n = 5) and is applied to its determination in spiked buckwheat samples using the standard addition method, with 96.9 ± 2.0% recovery. It is also demonstrated that NAD(P)H and NADH have different kinetics for AuNP generation, which could be used to discriminate between these two cofactors.
报道了由NADPH原位无种子形成金纳米颗粒及其在谷物中阿托品（一种托烷生物碱）比色测定中的应用。该方法基于化学水解，然后在Au(III)或Au(III)/Pt(II)存在下，由托品酮还原酶催化NADP进行酶促氧化。在该反应过程中，生成的NADPH将金属离子前体还原为AuNP（或AuPtNP），并测量由于等离子体带（550 nm或575 nm）引起的吸收。该方法（AuPtNP）能够在0.025至0.09 mM的浓度范围内测定分析物，相对标准偏差为3%（n = 5），并使用标准加入法应用于加标荞麦样品中的测定，回收率为96.9±2.0%。还证明了NAD(P)H和NADH在生成AuNP方面具有不同的动力学，这可用于区分这两种辅酶。
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