Song Qingting, Zhang Hailong, Kong Jia, Wong Man Shing, Li Hung-Wing
Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, SAR, China.
Department of Chemistry, Hong Kong Baptist University, Hong Kong, SAR, China.
Mikrochim Acta. 2025 Feb 4;192(3):131. doi: 10.1007/s00604-025-06995-4.
Neurodegenerative diseases (NDs) pose significant challenges to both longevity and quality of life, affecting millions worldwide and ranking as a major cause of global morbidity and disability. There is an urgent need for less invasive, cost-effective diagnostic methods that can reliably detect ND-related biomarkers in readily available biofluids such as serum. In response to this need, we have developed an ultra-sensitive assay that utilizes magnetic nanoparticles and a novel, tailor-made turn-on fluorescent probe named F-SPG. This innovative assay enables the detection of neurofilament light chain (NfL) at femtomolar concentrations, with a remarkable detection limit of 24 fM which is at least 18 times more sensitive than the conventional ELISA kits. Our assay's design eliminates the need for traditional secondary antibody in immunoassay, thereby streamlining the diagnostic process. Recoveries exceeding 95% underscore the assay's precision and reliability. It has proven effective in distinguishing Alzheimer's Disease patients from healthy individuals by quantifying serum NfL levels, showcasing its potential as a cost-effective, ultra-sensitive tool for the early screening of neurodegenerative diseases.
神经退行性疾病（NDs）对寿命和生活质量构成了重大挑战，影响着全球数百万人，是全球发病和残疾的主要原因之一。迫切需要侵入性较小、成本效益高的诊断方法，以便能够在血清等易于获取的生物流体中可靠地检测与ND相关的生物标志物。为满足这一需求，我们开发了一种超灵敏检测方法，该方法利用磁性纳米颗粒和一种新型的、量身定制的开启式荧光探针F-SPG。这种创新的检测方法能够检测飞摩尔浓度的神经丝轻链（NfL），其显著的检测限为24 fM，比传统酶联免疫吸附测定试剂盒至少灵敏18倍。我们检测方法的设计无需免疫测定中传统的二抗，从而简化了诊断过程。回收率超过95%突出了该检测方法的精密度和可靠性。通过量化血清NfL水平，该方法已被证明能有效区分阿尔茨海默病患者和健康个体，展示了其作为一种具有成本效益的超灵敏工具用于神经退行性疾病早期筛查的潜力。
