Khoury Pierre, Kharouf Naji, Etienne Olivier, Dillenseger Jean-Philippe, Haikel Youssef, El-Damanhoury Hatem M, Irani Dani, Ozcan Mutlu, Salameh Ziad
Department of Prosthodontics, Faculty of Dental Medicine, Lebanese University, Beirut, Lebanon.
Faculty of Dental Medicine, University of Strasbourg; Strasbourg, France.
J Contemp Dent Pract. 2024 Nov 1;25(11):1001-1008. doi: 10.5005/jp-journals-10024-3781.
To evaluate and compare the surface morphology, wettability, roughness, and bacterial adhesion properties of polymethyl methacrylate (PMMA) materials fabricated by conventional methods and 3D printing for complete denture applications.
Two PMMA materials were investigated: Conventionally processed (ProBase Hot) and 3D-printed (3DP) (V-Print Dentbase). Surface morphology ( = 3) was analyzed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Surface roughness ( = 10) was measured using an optical profilometer. Wettability was assessed through contact angle measurements ( = 6) at 10, 30, and 60 seconds. Bacterial adhesion ( = 9) and biofilm formation ( = 3) were evaluated using () as a model organism, with quantitative bacterial counts and SEM analysis of bacterial morphology. Data were statistically analyzed.
Scanning electron microscopy analysis revealed nanoparticles on the surface of 3DP samples, while EDX detected silicon in these samples, absent in conventional PMMA. 3D-printed surfaces exhibited significantly lower roughness (1.05 ± 0.32 µm) compared to conventional surfaces (20.46 ± 6.71 µm) ( < 0.001). Contact angle measurements showed that 3DP surfaces were more hydrophilic (64-68°) than conventional surfaces (100°) ( < 0.05). Bacterial adhesion studies demonstrated more adherent bacteria on 3DP surfaces (92.5 ± 30.8) compared to the conventional surfaces (57.6 ± 12.5), but biofilm formation was observed only on conventional surfaces.
3D-printed PMMA exhibited distinct surface characteristics compared to conventionally processed PMMA, including the presence of silicon nanoparticles, lower surface roughness, and higher hydrophilicity. While 3DP surfaces showed higher initial bacterial adherence, in contrast, they appeared to inhibit biofilm formation, which highlights the complex nature of bacterial interactions with these materials.
Further clinical studies are needed to validate the results of this investigation and generate clinical translational data. How to cite this article: Khoury P, Kharouf N, Etienne O, Physicochemical Properties and Bacterial Adhesion of Conventional and 3D Printed Complete Denture PMMA Materials: An Study - Part I. J Contemp Dent Pract 2024;25(11):1001-1008.
评估并比较通过传统方法和3D打印制造的用于全口义齿的聚甲基丙烯酸甲酯（PMMA）材料的表面形态、润湿性、粗糙度和细菌粘附特性。
研究了两种PMMA材料：传统加工的（ProBase Hot）和3D打印的（3DP）（V-Print Dentbase）。使用扫描电子显微镜（SEM）和能量色散X射线光谱仪（EDX）分析表面形态（n = 3）。使用光学轮廓仪测量表面粗糙度（n = 10）。通过在10秒、30秒和60秒时测量接触角（n = 6）来评估润湿性。使用金黄色葡萄球菌作为模式生物评估细菌粘附（n = 9）和生物膜形成（n = 3），包括定量细菌计数和对细菌形态的SEM分析。对数据进行统计学分析。
扫描电子显微镜分析显示3DP样品表面有纳米颗粒，而EDX在这些样品中检测到硅，传统PMMA中不存在。与传统表面（20.46 ± 6.71 µm）相比，3D打印表面的粗糙度显著更低（1.05 ± 0.32 µm）（P < 0.001）。接触角测量表明，3DP表面比传统表面更亲水（64 - 68°）（100°）（P < 0.05）。细菌粘附研究表明，与传统表面（57.6 ± 12.5）相比，3DP表面有更多的粘附细菌（92.5 ± 30.8），但仅在传统表面观察到生物膜形成。
与传统加工的PMMA相比，3D打印的PMMA表现出不同的表面特性，包括存在硅纳米颗粒、更低的表面粗糙度和更高的亲水性。虽然3DP表面显示出更高的初始细菌粘附，但相比之下，它们似乎抑制了生物膜形成，这突出了细菌与这些材料相互作用的复杂性。
需要进一步的临床研究来验证本研究结果并生成临床转化数据。如何引用本文：Khoury P, Kharouf N, Etienne O, 传统和3D打印全口义齿PMMA材料的物理化学性质和细菌粘附：一项体外研究 - 第一部分。《当代牙科实践杂志》2024；25（11）：1001 - 1008。
