Arslan Ezgi, Akay Canan, Erdönmez Demet, Avukat Esra Nur
Department of Prosthodontics, Faculty of Dentistry, Eskisehir Osmangazi University, Eskisehir, Türkiye.
Advanced Material Technologies Application and Research Center, Osmangazi University, Eskisehir, Türkiye.
BMC Oral Health. 2025 Mar 7;25(1):356. doi: 10.1186/s12903-025-05700-4.
The aim of this in vitro study was to evaluate the surface roughness and Candida albicans (C. albicans) biofilm formation of conventional heat-cured and different CAD/CAM milling denture base materials, after immersion in different pH levels of artificial saliva.
Disc-shaped samples (10 × 2 mm) (n = 10) were prepared from two brands of CAD/CAM milled and conventional heat-cured PMMA denture base materials. The samples were immersed at 37 °C for 21 days in different pH levels of artificial saliva. The surface roughness (Ra) of each sample was measured using a contact profilometer before and after aging. C. albicans adhesion of the samples was determined using the Crystal Violet (CV) method.
A significant increase in roughness was observed across all material groups maintained at pH 3 (P < 0.001). At pH 7, the roughness for heat-cured PMMA and G-PMMA groups increased significantly. Although pH 14 increased roughness in all groups, this was not significant. Upon comparison of roughness values post-aging, no significant differences were noted between the groups at pH 3 and pH 7; however, the G-PMMA group exhibited significantly lower surface roughness at pH 14 (P < 0.05). Among all material groups, the highest adhesion of C. albicans was observed in the heat-cured PMMA group. (P < 0.05).
Aging at different pH values caused surface changes in denture base materials and differences in C. albicans adhesion. Although there is an increase in surface roughness in all groups, these values are lower than the plaque accumulation threshold. The highest C. albicans adhesion was observed in heat-cured PMMA samples. Considering both roughness values and Candida adhesions, it is concluded that the new generation denture base materials are suitable for clinical use and should be widely used.
本体外研究旨在评估传统热固化及不同计算机辅助设计/计算机辅助制造（CAD/CAM）铣削义齿基托材料在浸入不同pH值的人工唾液后其表面粗糙度及白色念珠菌生物膜的形成情况。
从两个品牌的CAD/CAM铣削及传统热固化聚甲基丙烯酸甲酯（PMMA）义齿基托材料制备圆盘形样本（10×2mm）（n = 10）。将样本在37℃下于不同pH值的人工唾液中浸泡21天。使用接触式轮廓仪在老化前后测量每个样本的表面粗糙度（Ra）。采用结晶紫（CV）法测定样本的白色念珠菌黏附情况。
在pH值为3的所有材料组中均观察到粗糙度显著增加（P < 0.001）。在pH值为7时，热固化PMMA组和G-PMMA组的粗糙度显著增加。尽管pH值为14时所有组的粗糙度均增加，但不显著。在比较老化后的粗糙度值时，pH值为3和pH值为7的组之间未观察到显著差异；然而，G-PMMA组在pH值为14时表面粗糙度显著较低（P < 0.05）。在所有材料组中，热固化PMMA组观察到白色念珠菌的黏附性最高（P < 0.05）。
在不同pH值下老化会导致义齿基托材料表面变化及白色念珠菌黏附差异。尽管所有组的表面粗糙度均增加，但这些值低于菌斑积聚阈值。在热固化PMMA样本中观察到最高的白色念珠菌黏附性。综合粗糙度值和念珠菌黏附情况，得出新一代义齿基托材料适用于临床使用且应广泛应用的结论。
