硝基呋喃衍生物对菌株生物膜的有效性及其毒性。
Efficacy of nitrofuran derivatives against biofilms of strains and their toxicity.
作者信息
Vaso Carolina Orlando, Bila Níura Madalena, da Silva Rosângela Aparecida Moraes, de Carvalho Angélica Romão, Belizário Jennyfie Araújo, Pandolfi Fabiana, De Vita Daniela, Bortolami Martina, Mendes-Giannini Maria José Soares, Scipione Luigi, Di Santo Roberto, Costi Roberta, Costa-Orlandi Caroline Barcelos, Fusco-Almeida Ana Marisa
机构信息
Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, Brazil.
Department of Public and Animal Health, School of Veterinary, University Eduardo Mondlane (UEM), Maputo, Mozambique.
出版信息
Future Microbiol. 2025 Mar;20(4):305-314. doi: 10.1080/17460913.2025.2457286. Epub 2025 Feb 5.
AIM
To evaluate the efficacy of two nitrofuran derivatives against biofilms formed by two strains of and to study the toxicity of these compounds in alternative models: , , and zebrafish.
METHODS
The metabolic activity of biofilms was measured after treatment using the XTT reduction assay. Scanning electron microscopy (SEM) and confocal microscopy were used to observe damage to mature biofilms. Survival curves were generated for , while percentage survival was determined for and zebrafish.
RESULTS
The compounds showed efficacy against early and mature biofilms at concentrations equal to or up to two times higher than those required to eliminate planktonic fungal cells (3.90 to 31.25 μg/mL). Micrographs showed a reduction in metabolic activity, biofilm thickness, and extracellular matrix. In addition, the compounds showed little or no toxicity in alternative models, even at the highest concentrations tested.
CONCLUSION
These results are promising for the development of new therapeutic alternatives, especially for species, such as , which are recognized as high-priority pathogens. Few studies have investigated resistance and antifungal treatment targeting biofilms of this species, making this work a relevant contribution to future approaches.
目的
评估两种硝基呋喃衍生物对两种菌株形成的生物膜的疗效，并在替代模型（秀丽隐杆线虫、果蝇和斑马鱼）中研究这些化合物的毒性。
方法
使用XTT还原试验在处理后测量生物膜的代谢活性。采用扫描电子显微镜（SEM）和共聚焦显微镜观察成熟生物膜的损伤情况。绘制秀丽隐杆线虫的生存曲线，同时测定果蝇和斑马鱼的存活率。
结果
这些化合物在浓度等于或高达消除浮游真菌细胞所需浓度的两倍（3.90至31.25μg/mL）时，对早期和成熟生物膜均显示出疗效。显微照片显示代谢活性、生物膜厚度和细胞外基质均有所降低。此外，这些化合物在替代模型中即使在测试的最高浓度下也显示出很少或没有毒性。
结论
这些结果为开发新的治疗选择带来了希望，特别是对于被视为高优先级病原体的物种，如白色念珠菌。很少有研究调查该物种生物膜的耐药性和抗真菌治疗，因此这项工作对未来的研究方法具有重要贡献。
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