Peptides from against spp: toxicity in three-dimensional cell cultures and .

This work aimed to test peptides against the planktonic and biofilm form of spp. and toxicity using three-dimensional (3D) cells characterized and evaluate toxicity in . Susceptibility tests were conducted on the planktonic form and biofilm formation. The toxicity of the peptides was evaluated in lung and brain cells in monolayer (2D) and 3D mono- and co-culture, in addition to analysis with . Susceptibility values ranged from 31.25 to over 250 µg/ml with a fungicidal profile. Regarding toxicity, the PepM2 peptide was not toxic in 3D culture (500 µg/ml). , showed a survival rate of more than 85% In assays with brain and lung cell lines, concentrations ranged from 4 × 10 to 4 × 10 cells/well for brain cells and 1 × 10 cells/well for lung cells. Cocultures used 1 × 10 brain and 1 × 10 lung cells. This study shows that the peptides have great potential against cryptococcosis, and all spheroids were characterized as having a spheroidal and compact structure.