De Lima Gualque Marcos William, Vaso Carolina Orlando, Dos Santos Kelvin Sousa, Galeane Mariana Cristina, Gomes Paulo César, Palma Mario Sérgio, Soares Mendes Giannini Maria José, Moroz Andrei, Fusco Almeida Ana Marisa
Laboratory of Mycology & Center of Proteomics, Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, 14800-903, Brazil.
Laboratory for Monoclonal Antibodies, Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Araraquara, 14800-903, Brazil.
Future Microbiol. 2025 Jan;20(1):11-21. doi: 10.1080/17460913.2024.2421632. Epub 2024 Nov 18.
This work aimed to test peptides against the planktonic and biofilm form of spp. and toxicity using three-dimensional (3D) cells characterized and evaluate toxicity in . Susceptibility tests were conducted on the planktonic form and biofilm formation. The toxicity of the peptides was evaluated in lung and brain cells in monolayer (2D) and 3D mono- and co-culture, in addition to analysis with . Susceptibility values ranged from 31.25 to over 250 µg/ml with a fungicidal profile. Regarding toxicity, the PepM2 peptide was not toxic in 3D culture (500 µg/ml). , showed a survival rate of more than 85% In assays with brain and lung cell lines, concentrations ranged from 4 × 10 to 4 × 10 cells/well for brain cells and 1 × 10 cells/well for lung cells. Cocultures used 1 × 10 brain and 1 × 10 lung cells. This study shows that the peptides have great potential against cryptococcosis, and all spheroids were characterized as having a spheroidal and compact structure.
这项工作旨在使用具有特征的三维（3D）细胞测试肽对隐球菌属的浮游形式和生物膜形式的抗菌活性及其毒性，并评估其在小鼠体内的毒性。对浮游形式和生物膜形成进行了药敏试验。除了用小鼠进行分析外，还在单层（2D）以及3D单培养和共培养的肺细胞和脑细胞中评估了肽的毒性。药敏值范围为31.25至超过250μg/ml，具有杀菌特性。关于毒性，PepM2肽在3D培养中（500μg/ml）无毒。在脑和肺细胞系的试验中，脑细胞的浓度范围为每孔4×10⁵至4×10⁶个细胞，肺细胞为每孔1×10⁶个细胞。共培养使用1×10⁵个脑细胞和1×10⁵个肺细胞。这项研究表明，这些肽对隐球菌病具有很大的潜力，并且所有球体均具有球形和紧密结构的特征。
