Giretová Mária, Medvecký Ľubomír, Demčišáková Zuzana, Luptáková Lenka, Petrovová Eva, Štulajterová Radoslava
Division of Functional and Hybrid Materials, Institute of Materials Research of SAS, Košice, Slovakia.
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.
Front Cell Dev Biol. 2025 Jan 21;12:1504268. doi: 10.3389/fcell.2024.1504268. eCollection 2024.
The aim of this paper was to evaluate the effect of gel addition to biopolymeric scaffolds on the pro-angiogenic and basic material characteristics of the final composite for use in regenerative medicine. The studied scaffold consisted of natural biopolymers: polyhydroxybutyrate, chitosan, agarose, and gelatin. The final scaffold was characterized by high macroporosity (90%) and wide pore size distribution. As is known, the pore size is a critical factor for cell ingrowth in grafts after implantation in the body and for angiogenic development and creation of new vessels. After 9 days of cultivation in the culture medium, the scaffold retained its physicochemical properties without any tendency of disintegration. The addition of polymeric gels to the scaffold improved the mechanical stability of the composite. cytotoxicity testing showed good adherence of the seeded L929 fibroblasts on the scaffold and strong ingrowth of cells into the macropores. No sign of cytotoxicity was identified by both the MTS assay and live/dead cell staining. The quail chorioallantoic membrane (CAM) assay-as an alternative to assays-revealed suitable pro-angiogenic properties of the scaffold for the formation and ingrowth of new blood vessels. Moreover, the upregulation of gene expression responsible for the activation of angiogenic cascade clearly demonstrated a positive effect of the prepared composites on angiogenesis as an essential part of new tissue formation and the regeneration process itself.
本文旨在评估向生物聚合物支架中添加凝胶对用于再生医学的最终复合材料的促血管生成特性和基本材料特性的影响。所研究的支架由天然生物聚合物组成：聚羟基丁酸酯、壳聚糖、琼脂糖和明胶。最终的支架具有高孔隙率（90%）和较宽的孔径分布。众所周知，孔径是植入体内后移植物中细胞向内生长以及血管生成和新血管形成的关键因素。在培养基中培养9天后，支架保留了其物理化学性质，没有任何崩解的趋势。向支架中添加聚合物凝胶提高了复合材料的机械稳定性。细胞毒性测试表明，接种的L929成纤维细胞在支架上良好附着，并且细胞大量向内生长进入大孔。MTS 测定法和活/死细胞染色均未发现细胞毒性迹象。鹌鹑绒毛尿囊膜（CAM）试验——作为一种替代试验——揭示了该支架对于新血管形成和向内生长具有合适的促血管生成特性。此外，负责激活血管生成级联反应的基因表达上调清楚地证明了所制备的复合材料对血管生成具有积极作用，而血管生成是新组织形成和再生过程本身的重要组成部分。
