Xu Yaqi, Zhang Zhao, Bian Yanqing, Wang Yuanhao, Deng Ziliang, Luo Rui, Li Weijia, Yan Jingyi, Zhao Baohua, Sun Dongzhe
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Collaborative Innovation Center for Eco-Environment, Hebei Research Center of the Basic Discipline of Cell Biology, Biology Postdoctoral Research Station of Hebei Normal University, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
Hebei Ruilong Biotechnology Co., Ltd., Shijiazhuang 050024, China.
J Agric Food Chem. 2025 Feb 19;73(7):4137-4151. doi: 10.1021/acs.jafc.4c12478. Epub 2025 Feb 5.
sp. ATCC 20888 is an important species for industrial polyunsaturated fatty acids (PUFA) production. This study investigated the regulatory mechanisms affecting the proportions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in terms of biosynthesis and storage distribution. EPA and DHA possessed different accumulation patterns: EPA proportion increased over time, while DHA peaked at 48 h. EPA was predominantly integrated into triacylglycerol during the logarithmic phase and phosphatidylcholine during the stationary phase. Transcriptome analysis revealed that EPA synthesis involved the fatty acid synthase-elongase/desaturase system, while DHA depended mainly on PUFA synthase. Key enzymes, including elongase ELOVL7, diacylglycerol acyltransferase (g10562), and lysophosphatidylcholine acyltransferases (g8836 and g7540), show a positive correlation with EPA yield, highlighting their roles in its biosynthesis and storage. Additionally, phosphopantetheine adenylyl transferase (PPAT/COASY) and ADP-ribosylation factor 1_2 (ARF1_2) were identified as potential regulators of PUFA proportions. This study provided insights for genetic optimization of PUFA production in.
嗜热栖热菌ATCC 20888是工业生产多不饱和脂肪酸（PUFA）的重要菌种。本研究从生物合成和储存分布方面探究了影响二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）比例的调控机制。EPA和DHA具有不同的积累模式：EPA比例随时间增加，而DHA在48小时达到峰值。EPA在对数期主要整合到三酰甘油中，在稳定期则整合到磷脂酰胆碱中。转录组分析表明，EPA合成涉及脂肪酸合酶-延长酶/去饱和酶系统，而DHA主要依赖于PUFA合酶。包括延长酶ELOVL7、二酰基甘油酰基转移酶（g10562）和溶血磷脂酰胆碱酰基转移酶（g8836和g7540）在内的关键酶与EPA产量呈正相关，突出了它们在其生物合成和储存中的作用。此外，磷酸泛酰巯基乙胺腺苷酰转移酶（PPAT/COASY）和ADP-核糖基化因子1_2（ARF1_2）被确定为PUFA比例的潜在调节因子。本研究为PUFA生产的基因优化提供了见解。
