Mołoń Mateusz, Małek Gabriela, Bzducha-Wróbel Anna, Kula-Maximenko Monika, Mołoń Agnieszka, Galiniak Sabina, Skrzypiec Krzysztof, Zebrowski Jacek
Faculty of Biology and Nature Protection, Rzeszów University, 35-601, Rzeszów, Poland.
Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences, Ul. Nowoursynowska 159C, 02-776, Warsaw, Poland.
Biogerontology. 2025 Feb 5;26(2):54. doi: 10.1007/s10522-025-10196-0.
Aging is a multifactorial process that significantly impairs organismal function. Yeast is one of the model organisms used in aging research. Our understanding of the impact of the cell wall on aging remains elusive. Yeast cell wall is a complex and dynamic structure that plays a crucial role in the growth, survival, and aging of Saccharomyces cerevisiae. In this study, we demonstrated for the first time that the deletion of genes involved in cell wall biogenesis leads to significant impact on aging. In this study, we analysed five deletion mutants: crh2Δ, cwp1Δ, flo11Δ, gas1Δ and hsp12Δ. We showed a correlation between Raman spectroscopy signatures assigned to proteins, nucleic acids and RNA and replicative aging. Using Raman spectroscopy, we also revealed that a lack GAS1 gene results in significant changes in the biochemical composition of the cells that may increase sensitivity to environmental stressors. Our data unequivocally indicate that employing yeast as a model in aging research is appropriate, as long as the factors under analysis are not implicated in cell wall biogenesis.
衰老 是一个显著损害机体功能的多因素过程。酵母是衰老研究中使用的模式生物之一。我们对细胞壁对衰老影响的理解仍然难以捉摸。酵母细胞壁是一种复杂且动态的结构，在酿酒酵母的生长、存活和衰老中起着关键作用。在本研究中，我们首次证明参与细胞壁生物合成的基因缺失会对衰老产生重大影响。在本研究中，我们分析了五个缺失突变体：crh2Δ、cwp1Δ、flo11Δ、gas1Δ和hsp12Δ。我们展示了分配给蛋白质、核酸和RNA的拉曼光谱特征与复制性衰老之间的相关性。使用拉曼光谱，我们还发现缺乏GAS1基因会导致细胞生化组成发生显著变化，这可能会增加对环境应激源的敏感性。我们的数据明确表明，只要所分析的因素与细胞壁生物合成无关，将酵母用作衰老研究的模型就是合适的。
