Utilizing carboxymethyl cellulose to assist soy protein isolate in the formation of emulsion to deliver β-carotene: Exploring the correlation between interfacial behavior and emulsion stability.

This study investigated the effects of carboxymethyl cellulose (CMC) adsorption on the interfacial properties of soy protein isolate (SPI) and its correlation with emulsion stability. The findings revealed that SPI-CMC emulsions exhibited reduced zeta potential and particle size compared with SPI emulsion alone. Molecular docking analysis suggested that the enhanced stability of SPI-CMC emulsions was primarily due to hydrogen bonding and electrostatic interactions between SPI and CMC. Notably, the encapsulation efficiency of β-carotene in SPI-CMC emulsions increased by 47.74 % at pH 4.0 with 0.4 % CMC and by 39.55 % at pH 5.0 with 0.5 % CMC compared to SPI emulsion. Stability analyses demonstrated that at pH 4.0, the SPI-CMC interfacial layer formed by hydrogen bonding and electrostatic interactions effectively protected β-carotene from external degradation factors. At pH 5.0, steric hindrance facilitated the formation of a SPI-CMC network structure, increasing the path length for oxidants to reach the oil droplet interface. These distinct binding mechanisms in SPI-CMC emulsions effectively prolonged oil droplet digestion and regulated the release of free fatty acids. The resulting emulsion exhibited slow and sustained lipid release and digestion kinetics, making it a suitable model for designing sustained-release nutritional supplements.