Amini Mohammad Amin, Khodadadi Iraj, Tavilani Heidar, Abbasalipourkabir Roghayeh, Azizi Mehdi, Rashidi Khodabakhsh, Samadian Hadi, Karimi Jamshid
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Cancer Research Center, Institute of Cancer, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Biol Macromol. 2025 Apr;303:140569. doi: 10.1016/j.ijbiomac.2025.140569. Epub 2025 Feb 3.
This study developed a gelatin/alginate-based nanocomposite hydrogel (NC gel), incorporating selenium-doped deferoxamine-derived carbon quantum dots (Se.DFO-CQDs). Initially, Se.DFO-CQDs were synthesized and characterized through several tests, and subsequently, NC gels were created using an dual crosslinking method and analyzed through characterization tests such as SEM, EDX, FT-IR, XRD, tensile strength, water uptake, water vapor transmission rate, weight loss, porosity, blood compatibility, microbial penetration, and DPPH. In vivo studies revealed that NC gels containing Se.DFO-CQDs at 50 % and 0 % exhibited higher wound closure percentages than the control group. The highest wound closure percentage was observed in NC gels with Se.DFO-CQDs at 50 %, reaching 85.7 ± 3.98 % on the 7th day and 98.1 ± 3.95 % on the 14th day. Histological examinations demonstrated that NC gels with Se.DFO-CQDs at 50 % promoted more significant neovascularization, re-epithelialization, and collagen synthesis. Additionally, RT-qPCR results indicated that NC gels with Se.DFO-CQDs at 50 % significantly upregulated the mRNA expression of VEGF-A, bFGF, PDGF-b, and lncRNA GAS5 on the 7th day and COL1A1 on the 14th day. In conclusion, our findings suggest that the NC gels with Se.DFO-CQDs at 50 % show promise for enhancing wound healing and skin regeneration, potentially offering clinical applications.
本研究开发了一种基于明胶/藻酸盐的纳米复合水凝胶（NC凝胶），其中掺入了硒掺杂的去铁胺衍生碳量子点（Se.DFO-CQDs）。首先，通过多项测试对Se.DFO-CQDs进行了合成和表征，随后，采用双重交联法制备了NC凝胶，并通过扫描电子显微镜（SEM）、能量散射X射线光谱（EDX）、傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、拉伸强度、吸水率、水蒸气透过率、失重、孔隙率、血液相容性、微生物渗透性和二苯基苦味酰基自由基（DPPH）等表征测试对其进行了分析。体内研究表明，含有50%和0% Se.DFO-CQDs的NC凝胶的伤口闭合率高于对照组。在含有50% Se.DFO-CQDs的NC凝胶中观察到最高的伤口闭合率，在第7天达到85.7±3.98%，在第14天达到98.1±3.95%。组织学检查表明，含有50% Se.DFO-CQDs的NC凝胶促进了更显著的新血管形成、再上皮化和胶原蛋白合成。此外，逆转录定量聚合酶链反应（RT-qPCR）结果表明，含有50% Se.DFO-CQDs的NC凝胶在第7天显著上调了血管内皮生长因子A（VEGF-A）、碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子β（PDGF-b）和长链非编码RNA生长停滞特异性转录本5（lncRNA GAS5）的mRNA表达，在第14天上调了I型胶原蛋白α1链（COL1A1）的mRNA表达。总之，我们的研究结果表明，含有50% Se.DFO-CQDs的NC凝胶在促进伤口愈合和皮肤再生方面具有潜力，可能具有临床应用价值。
