Peng Yuxuan, Demidchik Vadim, Li Yan, Shen Zhenguo
Hainan College of Vocation and Technique, 95 Nanhai Ave., 570100 Haikou, China; Department of Plant Cell Biology and Bioengineering, Biology Faculty, Belarusian State University, 4 Independence Ave., 220030 Minsk, Belarus.
Institute of Experimental Botany, National Academy of Sciences of Belarus, 27 Botanichskaya St., 220072 Minsk, Belarus; International Research Centre for Environmental Membrane Biology and Department of Horticulture, Foshan University, Foshan, China.
Fitoterapia. 2025 Apr;182:106411. doi: 10.1016/j.fitote.2025.106411. Epub 2025 Feb 3.
Terpenoids are important components that exert pharmacological effects in Rubiaceae plants. In this study, two Rubiaceae plants, Nauclea officinalis and Paederia scandens, which are widely distributed in Hainan, China, were collected. The extracts of these two plants were obtained through boiling water extraction and analyzed using UHPLC-ESI-QE-Orbitrap-MS. By comparing with the mzCloud database, terpenoids with a matching rate of 85 % were identified. The results revealed that the aqueous extracts of N. officinalis mainly contain six pentacyclic triterpenoids, one diterpenoid, and one iridoid. The aqueous extracts of P. scandens contains one monoterpenoid, one diterpenoid, two pentacyclic triterpenes, and 4 iridoids. To verify the anti-inflammatory efficacy of the two extracts, in this study, they were added to the lipopolysaccharide (LPS)-induced RAW264.7 macrophages inflammation model. The results showed that the two extracts can reduce the secretion of proinflammatory cytokines IL-1β, IL-6, and TNF-α (p < 0.05) and the mortality rate of cell inflammation (p < 0.05) by inhibiting the activation of the NF-κB/NLRP3 pathway (p < 0.05). The study identified the terpenoids in N. officinalis and P. scandens and verified their anti-inflammatory effects in the RAW264.7 macrophages inflammation model.
萜类化合物是茜草科植物中发挥药理作用的重要成分。在本研究中，采集了两种广泛分布于中国海南的茜草科植物，即乌檀和鸡矢藤。通过沸水提取获得这两种植物的提取物，并采用超高效液相色谱-电喷雾电离-四极杆-轨道阱-质谱联用仪（UHPLC-ESI-QE-Orbitrap-MS）进行分析。通过与mzCloud数据库进行比对，鉴定出匹配率为85%的萜类化合物。结果显示，乌檀的水提取物主要含有六种五环三萜、一种二萜和一种环烯醚萜。鸡矢藤的水提取物含有一种单萜、一种二萜、两种五环三萜和4种环烯醚萜。为验证这两种提取物的抗炎功效，在本研究中，将它们添加到脂多糖（LPS）诱导的RAW264.7巨噬细胞炎症模型中。结果表明，这两种提取物可通过抑制NF-κB/NLRP3通路的激活（p<0.05），降低促炎细胞因子IL-1β、IL-6和TNF-α的分泌（p<0.05）以及细胞炎症死亡率（p<0.05）。该研究鉴定了乌檀和鸡矢藤中的萜类化合物，并在RAW264.7巨噬细胞炎症模型中验证了它们的抗炎作用。
