Cho Yeon-Woo, Kang Min-Ji, Park Joon-Ha, Eom Yun-Sik, Kim Tae-Hyung
Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU), Suwon, Gyeonggi, 16419, Republic of Korea.
School of Integrative Engineering, Chung-Ang University, 84 Heukseuk-ro, Dongjak-gu, Seoul, 06974, Republic of Korea.
Nat Commun. 2025 Feb 6;16(1):1389. doi: 10.1038/s41467-025-56373-0.
Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and nanoline arrays fabricated through interference lithography and selectively capture of UiO-67 metal-organic frameworks on nanoline arrays with a 99.8% efficiency using an optimised asymmetric spin-coating method. The UiO-67 metal-organic frameworks contain three osteogenic differentiation factors for sustained release over four weeks. The combination of differentiation factors and patterned array allows for generation of adipocytes, osteoblasts, and adipocyte-osteoblast mixtures on nanohole arrays, nanoline arrays, and at the nanohole-nanoline interface, respectively, with mature osteoblasts exhibiting higher marker expression and mineralisation. The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models.
再生治疗和药物筛选需要改进的体外模型。在此，我们报告了功能对齐的纳米颗粒捕获纳米图案阵列，用于在单个基板上进行空间控制的精确间充质干细胞分化。这些阵列包括通过干涉光刻制造的纳米孔和纳米线阵列，并使用优化的不对称旋涂方法以99.8%的效率在纳米线阵列上选择性捕获UiO-67金属有机框架。UiO-67金属有机框架包含三种成骨分化因子，可在四周内持续释放。分化因子和图案化阵列的组合分别允许在纳米孔阵列、纳米线阵列以及纳米孔-纳米线界面上生成脂肪细胞、成骨细胞和脂肪细胞-成骨细胞混合物，成熟的成骨细胞表现出更高的标志物表达和矿化。这种持续释放的图案化阵列在构建先进的治疗和疾病状态体外细胞模型方面具有潜力。
