Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil.
School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Campinas, 13083-862, SP, Brazil.
J Bone Miner Metab. 2021 Mar;39(2):163-173. doi: 10.1007/s00774-020-01152-8. Epub 2020 Sep 5.
The jabuticaba peel extract (JPE) contains bioactive compounds that regulate fat metabolism. Because the negative correlation between fat accumulation and bone formation in bone marrow, we hypothesized that JPE inhibits adipocyte as well as favors osteoblast differentiation of mesenchymal stromal cells (MSCs) under healthy and osteoporotic conditions, a disease that display an imbalance between adipocyte and osteoblast differentiation resulting in reduced bone mass.
To test these hypotheses, bone marrow MSCs were harvested from healthy and osteoporotic rats and cultured in adipogenic and osteogenic media with three concentrations of JPE, 0.25, 5 and 10 µg/ml, and vehicle (control). After selecting the most efficient concentrations of JPE, we used them to evaluate adipocyte and osteoblast differentiation of MSCs from both sources.
We observed that, in general, JPE inhibited adipocyte differentiation of MSCs with more pronounced effects in cells from healthy than osteoporotic rats. In addition, JPE increased osteoblast differentiation, exhibiting a slightly higher osteogenic potential on MSCs from osteoporotic compared to healthy condition.
Our results demonstrated that JPE drives MSCs to inhibit adipocyte differentiation and toward osteoblast differentiation under healthy and osteoporotic conditions. These findings pave the way for further translational studies to investigate the therapeutic possibilities of JPE in both prevention and treatment of osteoporosis.
穗状醋栗果皮提取物 (JPE) 含有调节脂肪代谢的生物活性化合物。由于骨髓中脂肪堆积与成骨之间存在负相关，我们假设 JPE 不仅可以抑制脂肪细胞分化，还可以促进骨髓间充质基质细胞 (MSCs) 在健康和骨质疏松条件下向成骨细胞分化，这种疾病表现为脂肪细胞和成骨细胞分化失衡，导致骨量减少。
为了验证这些假设，我们从健康和骨质疏松大鼠的骨髓中分离出 MSCs，并在含有三种浓度 JPE（0.25、5 和 10μg/ml）和载体（对照）的成脂和成骨培养基中培养。在选择出最有效的 JPE 浓度后，我们用它们来评估来自两种来源的 MSCs 的脂肪细胞和成骨细胞分化。
我们观察到，一般来说，JPE 抑制 MSCs 的脂肪细胞分化，在来自健康大鼠的细胞中效果更为明显。此外，JPE 增加了成骨细胞分化，在骨质疏松症条件下对 MSCs 的成骨潜力略高于健康条件。
我们的研究结果表明，JPE 可在健康和骨质疏松条件下驱动 MSCs 抑制脂肪细胞分化并向成骨细胞分化。这些发现为进一步的转化研究铺平了道路，以研究 JPE 在预防和治疗骨质疏松症方面的治疗可能性。
